MAPKAPK-2 Antibody Sampler Kit

• 产品编号：CST-9329S      品牌：Cell Signaling Technology       原厂货号：9329S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

MAPKAPK-2 Antibody Sampler Kit中每个抗体都能检测它所对应的内源性靶点蛋白水平。活化状态的抗体只能检测所列物种中的磷酸化靶蛋白。MAPKAPK-2 Antibody不能与MAPKAPK-3 、MAPKAPK-5发生交叉反应。该多克隆抗体是采用合成的与人源MAPKAPK-2蛋白丝羧端残基相一致的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。该单克隆抗体是采用合成的与人源MAPKAPK-2蛋白Thr222和Thr334位点周围残基相一致的磷酸化肽段免疫动物后获得的。MAPKAPK-2 Antibody Sampler Kit为评估特定位点磷酸化的MAPKAPK-2蛋白水平，以及总MAPKAPK-2蛋白水平提供了一个经济的方法。该试剂盒所提供的一抗和二抗可以完成四次Western blot实验。在细胞因子、压力和化学因子的刺激下，丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点（1）。在体内，在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是，在体外激酶实验中，仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化（2）。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的（2）。Thr25可以在体外被p42 MAPK磷酸化，但是该磷酸化对于MAPKAPK-2的激活不是必须的（2）。

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

原厂资料：

Specificity / Sensitivity

Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 Antibody does not cross-react with MAPKAPK-3 or -5.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal region of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr222 and Thr334 of human MAPKAPK-2.

Description

The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Background

In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.