p90RSK Antibody Sampler Kit

• 产品编号：CST-9326S      品牌：Cell Signaling Technology       原厂货号：9326S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

RSK1/RSK2/RSK3 (32D7) Rabbit mAb兔单抗能检测内源性总RSK1-3蛋白水平。每个针对活化状态的单克隆抗体都能识别它的特异性靶点，并可能与相关磷酸化的RSK蛋白发生交叉反应。该磷酸特异性多克隆抗体通过使用与鼠源RSK1蛋白苏氨酸（359位点）/丝氨酸（363位点）和人源p90RSK蛋白周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。兔单克隆抗体通过使用与人源p90RSK蛋白丝氨酸（380位点）周围残基相一致的合成磷酸化肽段、人源p90RSK 蛋白包括RSK1/RSK2/RSK3蛋白的天冬氨酸（659位点）周围残基相一致的非磷酸化免疫动物而获得。p90RSK Antibody Sampler Kit提供了一个经济的方法评价特异位点磷酸化的p90RSK蛋白水平，以及总p90RSK蛋白水平。该试剂盒包含足够的一抗、二抗完成四个免疫印记实验。90kDa核糖体S6激酶(RSK1-4)是一个广泛表达的丝/苏氨酸激酶家族，其特征是包含2个不同的功能性激酶结构域（1）和一个羧基端的细胞外信号调节激酶（ERKs）结合位点（2）。RSK激酶结构域内或以外的一些位点，包括Ser380, Thr359, Ser363和Thr573，对于激酶的激活非常重要（3）。RSK1-3在多种生长因子、多肽激素和神经递质的刺激下，通过MAPKs的磷酸化作用、自磷酸化作用以及PI3K的信号途径的协同作用被激活（3）。

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

原厂资料：

Specificity / Sensitivity

RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1-3 proteins. Each activation state monoclonal antibody recognizes its specific target; some cross-reactivity with related phosphorylated RSK proteins may occur.

Source / Purification

The phospho-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of rat RSK1 and Thr573 of human p90RSK. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The rabbit monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser380 of human p90RSK and a nonphosphorylated peptide surrounding Asp659 of human p90RSK for total RSK1/RSK2/RSK3.

Description

The p90RSK Antibody Sampler Kit provides an economical means of evaluating levels of p90RSK protein phosphorylated at specific sites, as well as total p90RSK protein levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at -20°C. Do not aliquot the antibodies