GSK-3β-Tag Antibody

• 产品编号：CST-9325S      品牌：CST       原厂货号：9325S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

这个抗体可以检测出转染水平的GSK-3β标记的蛋白和内源性GSK-3β（46KDa）。多克隆抗体是通过用合成GSK-3β-tag的肽段免疫动物生产的。多克隆抗体是采用蛋白A和肽亲和色谱方法提纯的。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。磷酸化短肽标签如GSK-3β-tag，可以用于检测第三分泌系统效应蛋白的易位。

1. Hohlfeld, S. et al. (2006) Mol. Microbiol. 59, 1624-1637.

原厂资料：

Specificity / Sensitivity

GSK-3β-Tag Antibody detects transfected levels of GSK-3β-tagged proteins as well as endogenous GSK-3β (46 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1).

1. Hohlfeld, S. et al. (2006) Mol. Microbiol. 59, 1624-1637.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.