Src Family Antibody Sampler Kit

• 产品编号：CST-9320S      品牌：Cell Signaling Technology       原厂货号：9320S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Src Family Antibody Sampler Kit 中的每个抗体都能检测内源性靶蛋白水平，并且不能与其他家族成员发生交叉反应。该单克隆抗体是采用与人源Src蛋白胺端残基1-110相一致的GST-Src融合蛋白免疫动物后获得的。其他单克隆抗体是通过采用与人源Csk蛋白缬氨酸（399位点）、Lck蛋白苏氨酸（50位点）周围残基和Lyn蛋白胺端序列相一致的合成肽段免疫动物后获得的。该多克隆抗体是通过用与人源Fyn蛋白丝氨酸（25位点）周围残基和Yes蛋白胺端残基相一致的合成肽段免疫动物后获得的。该多克隆抗体经蛋白A和肽段亲和层析而纯化。Src Family Antibody Sampler Kit为评估Src家族成员蛋白总水平提供了快速、经济的方法。该试剂盒包含足够的一抗、二抗完成四次Western blot实验。Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的，但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化，能够上调酶活性；而蛋白羧基端尾部Tyr527位点被Csk磷酸化后，则会使酶的活性降低(2)。激酶Fyn在T细胞受体信号转导中起一定作用，也有助于记忆的形成和粘附分子调节的信号转导(3,4)。淋巴细胞特异性激酶Lck对T细胞分化和活化很重要(3)。造血Lyn激酶也能被Csk蛋白磷酸化，它涉及B细胞效应、转移和发育的调节(5)。广泛表达的激酶Yes在包括G蛋白偶联受体在内的多种细胞表面受体的下游起作用。同时它还涉及血管再生、细胞周期和细胞粘附的调节(6,7)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
4. Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
5. Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
6. Summy, J.M. et al. (2003) J. Cell Sci. 116, 2585-2598.
7. Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.

原厂资料：

Specificity / Sensitivity

Each antibody in the Src Family Antibody Sampler Kit detects endogenous levels of its target protein and does not cross-react with other family members.

Source / Purification

Src (32G6) Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing amino acid residues 1-110 of human Src. The remaining monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val399 of human Csk, Thr50 of human Lck, and the amino-terminal sequence of human Lyn. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser25 of human Fyn and the amino terminus of human Yes. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Src Family Antibody Sampler Kit provides an economical means of evaluating total levels of Src family member proteins. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
4. Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
5. Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
6. Summy, J.M. et al. (2003) J. Cell Sci. 116, 2585-2598.
7. Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA and 50% glycerol and less than
0.02% sodium azide. Store at –20°C. Do not aliquot the
antibodies