Phospho-Rb (Ser807/811) Antibody

• 产品编号：CST-9308S      品牌：CST       原厂货号：9308S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Rb (Ser807/811) Antibody 能够检测内源性丝氨酸（807/811位）磷酸化的Rb蛋白。该抗体也许与丝氨酸（608位）磷酸化的Rb蛋白烦死交叉反应。该多克隆抗体是由合成的人源的针对Rb蛋白丝氨酸（Ser807/811 位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8)，而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。

1. Sherr, C.J. (1996) Science 274, 1672-7.
2. Nevins, J.R. (1992) Science 258, 424-9.
3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-Rb (Ser807/811) Antibody detects endogenous levels of Rb when phosphorylated at serine 807/811. The antibody may cross-react with Rb phosphorylated at Ser608.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser807/811 of human Rb. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). 

1. Sherr, C.J. (1996) Science 274, 1672-7.
2. Nevins, J.R. (1992) Science 258, 424-9.
3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
4. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
5. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
7. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
8. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.