MNDA (3C1) Rat mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-9283S      品牌：Cell Signaling Technology       原厂货号：9283S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate) 抗体检测内源MNDA总蛋白水平。此单克隆抗体是通过用从人类粒性白细胞细胞核提取物免疫动物而获得。CST公司生产的此抗体与Alexa Fluor® 488荧光染料偶联，并通过人源细胞的流式细胞仪的测试。正如期望，结合了Alexa Fluor® 488的此抗体与未结合荧光染料的抗体一样呈现出同物种交叉反应。骨髓细胞核分化抗原(MNDA )是转录调控因子并在外周血粒性白细胞和单核细胞中组成型表达， 在一些B 细胞中也有低表达的情况(1,2)。MNDA 是干扰素(IFN)所调控的200个基因家族中的一员，它它包含了一个或几个部分保守的区域200个氨基酸残基，这些保守的区域被认为介导了蛋白-蛋白的相互作用(3)。 MNDA 在细胞凋亡中起了重要的作用，它在骨髓发育不良综合症 (MDS)中的表达是降低的(4)。 MNDA 被认为是节缘区淋巴瘤的标记物(5)。

1. Briggs, R.C. et al. (1994) J Cell Biochem 56, 559-67.
2. Miranda, R.N. et al. (1999) Hum Pathol 30, 1040-9.
3. Landolfo, S. et al. (1998) Biochimie 80, 721-8.
4. Briggs, R.C. et al. (2006) Cancer Res 66, 4645-51.
5. Kanellis, G. et al. (2009) Leukemia 23, 1847-57.

原厂资料：

Specificity / Sensitivity

MNDA (3C1) Rat mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total MNDA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a nuclear extract from human granulocytes.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated MNDA (3C1) Rat mAb #3329.

Background

MNDA (myeloid cell nuclear differentiation antigen) is a transcription factor constitutively expressed in peripheral blood granulocytes and monocytes; low expression is also found in a subset of B cells (1,2). MNDA is a member of the interferon (IFN)-regulated 200 family of genes, which contain one or more copies of a partially conserved domain of 200 amino acid residues thought to mediate protein-protein interaction (3). MNDA may play a role in apoptosis and its expression is reduced in myelodysplastic syndromes (MDS) (4). MNDA has been proposed to be a marker for nodal marginal zone lymphoma (5).

1. Briggs, R.C. et al. (1994) J Cell Biochem 56, 559-67.
2. Miranda, R.N. et al. (1999) Hum Pathol 30, 1040-9.
3. Landolfo, S. et al. (1998) Biochimie 80, 721-8.
4. Briggs, R.C. et al. (2006) Cancer Res 66, 4645-51.
5. Kanellis, G. et al. (2009) Leukemia 23, 1847-57.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.