PhosphoPlus? c-Jun (Ser63) and c-Jun (Ser73) Antibody Kit

• 产品编号：CST-9260S      品牌：CST       原厂货号：9260S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
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描述：

Phospho-c-Jun (Ser63) (54B3) Rabbit mAb兔单抗能够检测内源性Ser63位点磷酸化的c-Jun蛋白水平。Phospho-c-Jun (Ser73) Antibody兔多抗能够检测内源性Ser73位点磷酸化的c-Jun蛋白水平。该抗体也能识别Ser100位点磷酸化的JunD蛋白。c-Jun (60A8) Rabbit mAb兔单抗能够检测内源性c-Jun总蛋白水平。磷酸化多克隆抗体是采用合成的与人源c-Jun蛋白Ser63或者Ser73位点周围序列相一致的磷酸化肽段免疫兔后产生的，并经蛋白A和肽亲和层析纯化。单克隆抗体是采用合成的与人源c-Jun蛋白胺端序列相一致的肽段免疫兔后产生的。PhosphoPlus (R) c-Jun (Ser63)、c-Jun (Ser73) Antibody Kit提供了快速分析c-Jun蛋白磷酸化状态的试剂和对照。c-Jun是Jun家族的一个成员，Jun家族成员包括c-Jun, JunB, 和 JunD。c-Jun是转录因子激活蛋白-1（AP-1）的一个组分。AP-1是由Fos和Jun形成的二聚体以及 ATF家族成员组成的，能够结合并激活TRE/AP-1元件的转录（1）。包括生长因子，细胞因子和压力在内的细胞外信号能够激活AP-1依赖的转录过程。C-Jun的转录活性是通过SAPK/JNK对其中的Ser63和Ser73的磷酸化来调控的（2）。小鼠敲除实验表明c-Jun对于胚胎形成是至关重要的（3），进一步的研究发现c-Jun在多种组织和发育过程中发挥作用，包括轴突再生（4），肝脏再生（5）和T细胞发育（6）。AP-1所调控的基因发挥了多种生物学功能，包括细胞增殖、分化、凋亡、转化，侵润和转移，这依赖于细胞种类和具体情况（7-9）。其他的AP-1所调控的靶基因还能够调节细胞存亡，以及低氧和血管生成（8,10）。研究提示c-Jun可以作为癌症、血管重构、急性炎症反应和风湿性关节炎的重要治疗靶点（11,12）。

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

原厂资料：

Specificity / Sensitivity

Phospho-c-Jun (Ser63) (54B3) Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at Ser63. Phospho-c-Jun (Ser73) Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser73. This antibody also recognizes phosphorylation of JunD at Ser100. c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein.

Source / Purification

Phospho-specific polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser63 or Ser73 of human c-Jun, and purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal of human c-Jun.

Description

The PhosphoPlus (R) c-Jun (Ser63) and c-Jun (Ser73) Antibody Kit provided reagents and controls for rapid analysis of c-Jun phosphorylation status

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies