Phospho-ATF-2 (Thr69/71) Antibody

• 产品编号：CST-9225S      品牌：CST       原厂货号：9225S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-ATF-2 (Thr69/71) Antibody兔多抗能够检测内源性的Thr69和Thr71位点共磷酸化的ATF-2蛋白水平，但是不能识别Thr69位点或Thr71位点单独磷酸化的ATF-2蛋白。该多克隆抗体是采用合成的与人源ATF-2蛋白Thr69和Thr71位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。转录因子ATF-2（也被称为 CRE-BP1）能够结合到AP-1和 CRE DNA反应元件，是ATF/CREB亮氨酸拉链蛋白质家族的成员(1)。ATF-2蛋白能够调控多种病毒癌基因以及抑癌基因的表达，是SAPK/JNK 、p38 MAPK信号传导通路的靶标 (2-4)。多种细胞压力包括基因毒剂、炎性细胞因子和紫外辐射，都能激活ATF-2蛋白的转录活性。细胞应激刺激通过磷酸化Thr69和Thr71位点使ATF-2蛋白被激活 (2-4)。在体外以及转染了ATF-2的细胞中，SAPK、p38 MAPK蛋白都被证明能够磷酸化ATF-2蛋白的这些位点。这些位点的突变能导致应激性的ATF-2诱导转录活性降低(2-4)，并且降低E1A 、Rb蛋白通过ATF-2介导的基因表达。(2)。

1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
2. Gupta, S. et al. (1995) Science 267, 389-93.
3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

原厂资料：

Specificity / Sensitivity

Phospho-ATF-2 (Thr69/71) Antibody detects endogenous levels of ATF-2 only when dually phosphorylated at both threonine 69 and threonine 71. It does not recognize ATF-2 singly phosphorylated at either threonine 69 or threonine 71.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr69 and Thr71 of human ATF-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
2. Gupta, S. et al. (1995) Science 267, 389-93.
3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.