PhosphoPlus? p38 MAPK (Thr180/Tyr182) Antibody Kit

• 产品编号：CST-9210S      品牌：CST       原厂货号：9210S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
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描述：

Phospho-p38 MAPK Antibody兔多抗能够检测Thr180位点和Tyr182位点磷酸化的MAPK蛋白水平。该抗体不能与p42/44 MAPK、SAPK/JNK相应的磷酸化形式发生交叉反应。该单克隆抗体是采用合成的与人源p44 MAPK蛋白Thr180/Tyr182位点周围残基相一致的磷酸化肽段免疫动物后获得的。该多克隆抗体是采用合成的与人源p38 MAPK (p38 MAP Kinase Antibody)蛋白序列相一致的肽段免疫动物后获得的。p38 MAP kinase (MAPK),也被称为RK (1)或CSBP (2)，是酵母中HOG激酶在哺乳动物中的同源蛋白，在细胞因子和应急诱导的信号转导过程中起重要作用。(1-4)。已经发现了4种亚型的p38 MAPK，分别是p38α, β, γ（也被称为Erk6或 SAPK3）以及δ (也被称为 SAPK4)。与SAPK/JNK通路类似，p38 MAPK可以被多种细胞压力所激活，包括渗透压休克，炎症因子，脂多糖（LPS），紫外线照射和生长因子（1-5）。MKK3, MKK6和SEK能够使p38 MAPK的Thr180 和Tyr182位点磷酸化，从而激活p38 MAPK。激活的p38 MAPK能够磷酸化并激活MAPKAP kinase 2 (3)，进而最终磷酸化ATF-2 (5), Max (6)和MEF2等转录因子(5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制剂。该复合物通过抑制p38 MAPK及后续HSP27的磷酸化，抑制MAPKAPK-2的激活（9）。SB203580能够结合到p38 MAPK与ATP结合的位置从而抑制p38 MAPK的催化活性，但不会通过上游激酶抑制p38 MAPK的磷酸化（10）。

1. Rouse, J. et al. (1994) Cell 78, 1027-37.
2. Han, J. et al. (1994) Science 265, 808-11.
3. Lee, J.C. et al. (1994) Nature 372, 739-46.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

原厂资料：

Specificity / Sensitivity

Phospho-p38 MAPK Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAPK but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK (p38 MAP Kinase Antibody).

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). 
 SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

1. Rouse, J. et al. (1994) Cell 78, 1027-37.
2. Han, J. et al. (1994) Science 265, 808-11.
3. Lee, J.C. et al. (1994) Nature 372, 739-46.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.