CtIP (D76F7) Rabbit mAb

• 产品编号：CST-9201S      品牌：CST       原厂货号：9201S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CtIP (D76F7) Rabbit mAb能够检测内源性CtIP总蛋白。该单克隆抗体是由合成的人源的针对CtIP蛋白羧基末端的肽段免疫动物生产的。DNA损伤检验点对于损伤DNA修复和基因组维持的调节至关重要。CtIP/RBBP8 (CtBP-相互作用蛋白)，最初的特征是转录因子CtBP的结合伴侣，现已成为细胞周期进程和DNA双链断裂(DSB)修复的重要调节子。伴随DSB-传感 MRN复合体(MRE11-RAD50-NBS1)，CtIP在DSBs的单链DNA生成的过程中发挥作用，该过程为DNA修复机制信号所必需(回顾1)。CtIP被认为在DSBs传感和同源重组(HR)修复转换过程中起重要作用(2,3)。除HR之外，DSBs也可以通过非同源末端连接(NHEJ)进行修复， CtIP在NHEJ信号通路中发挥作用且不依赖其在DSB末端切除中的功能(4)。CtIP也参与细胞对拓扑异构酶抑制剂喜树碱和依托泊苷的耐受，从而利用其诱导周期细胞DSBs的能力来治疗癌症(5)。

1. You, Z. and Bailis, J.M. (2010) Trends Cell Biol 20, 402-9.
2. You, Z. et al. (2009) Mol Cell 36, 954-69.
3. Eid, W. et al. (2010) EMBO Rep 11, 962-8.
4. Quennet, V. et al. (2010) Nucleic Acids Res, Epub ahead of print.
5. Nakamura, K. et al. (2010) PLoS Genet 6, e1000828.

原厂资料：

Specificity / Sensitivity

CtIP (D76F7) Rabbit mAb recognizes endogenous levels of total CtIP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CtIP protein. 

Background

DNA damage checkpoints are critical for regulated repair of damaged DNA and genome maintenance. CtIP/RBBP8 (CtBP-interacting protein), initially characterized as a binding partner for the trancription factor CtBP, has emerged as a regulator of both cell cycle progression and repair of DNA double strand breaks (DSB). Along with the DSB-sensing MRN complex (MRE11-RAD50-NBS1), CtIP functions in the generation of single stranded DNA at DSBs, a process required for signaling to DNA repair machinery (reviewed in 1). CtIP is thought to be critical in the transition between sensing of DSBs and repair by homologous recombination (HR) (2,3). 
 
 In addition to HR, DSBs can also be repaired through nonhomologous end joining (NHEJ), and CtIP has been shown to have a role in signaling to the NHEJ pathway independently of its function in DSB end resection (4). 
 
 CtIP is also involved in cellular tolerence of topoisomerase inhibitors camptothecin and etoposide, which are used to treat cancer through their ability to introduce DSBs in cycling cells (5).

1. You, Z. and Bailis, J.M. (2010) Trends Cell Biol 20, 402-9.
2. You, Z. et al. (2009) Mol Cell 36, 954-69.
3. Eid, W. et al. (2010) EMBO Rep 11, 962-8.
4. Quennet, V. et al. (2010) Nucleic Acids Res, Epub ahead of print.
5. Nakamura, K. et al. (2010) PLoS Genet 6, e1000828.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody