Phospho-CREB (Ser133) (87G3) Rabbit mAb

• 产品编号：CST-9198S      品牌：CST       原厂货号：9198S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗可识别内源性的Ser133磷酸化的CREB。该抗体也可识别磷酸化的CREB相关蛋白ATF-1。该单克隆抗体由合成的人类CREB蛋白Ser133位点附近磷酸化肽段免疫动物而制成。CREB的是一个bZIP转录因子，通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号，调节很多的细胞反应。CREB的在许多组织中表达，它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用（1-3）。此外，CREB信号在数种生物体中涉及学习和记忆（4-6）。CREB能够通过与不同二聚体相互作用，选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活，激活可由多种信号通路完成，包括ERK，Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK，MSK，CaMKIV， MAPKAPK-2（7-9）。

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.
10. Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.
10. Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody