ACSL1 (D2H5) Rabbit mAb

• 产品编号：CST-9189S      品牌：CST       原厂货号：9189S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ACSL1 (D2H5) Rabbit mAb用于检测内源性ACSL1总蛋白水平。该单抗通过用与人ACSL1蛋白Ala257位点周围残基序列相同的合成肽免疫动物而得。哺乳类长链酰基辅酶A合成酶催化脂肪酸与辅酶A的连接反应，这是一个两步化学反应(1)。已经发现有5种ACSL的同工体(1)。这些同工体具有不同的底物偏好和亚细胞定位(1)。大鼠原始肝脏细胞ACSL1的过表达会导致其脂肪酸代谢的一些改变(2)。

1. Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
2. Li, L.O. et al. (2006) J Biol Chem 281, 37246-55.

原厂资料：

Specificity / Sensitivity

ACSL1 (D2H5) Rabbit mAb recognizes endogenous levels of total ACSL1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala257 of human ACSL1 protein.

Background

Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (1). Five isoforms of ACSL have been identified (1). These isoforms have different substrate preferences and subcellular localizations (1). Overexpression of ACSL1 results in changes to fatty acid metabolism in rat primary hepatocytes (2).

1. Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
2. Li, L.O. et al. (2006) J Biol Chem 281, 37246-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody