Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-9187S      品牌：CST       原厂货号：9187S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-CREB (Ser133) (87G3) (Alexa Fluor488 Conjugate) Rabbit mAb兔单抗识别内源性的Ser133磷酸化的CREB蛋白。该抗体也可识别磷酸化的CREB相关蛋白ATF-1。该单克隆抗体由合成的人类CREB蛋白Ser133位点附近磷酸化肽段免疫动物而制成。该抗体与Alexa Fluor® 488 在最优条件下偶联，F/P比率2-6。CST的此抗体与Alexa Fluor®488 荧光染料偶联，已通过用人和小鼠细胞进行直接流式分析和免疫荧光分析的内部测试。该抗体预计与未偶联的 Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗#9198 具有相同的种属交叉反应。CREB的是一个bZIP转录因子，通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号，调节很多的细胞反应。CREB的在许多组织中表达，它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用（1-3）。此外，CREB信号在数种生物体中涉及学习和记忆（4-6）。CREB能够通过与不同二聚体相互作用，选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活，激活可由多种信号通路完成，包括ERK，Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK，MSK，CaMKIV， MAPKAPK-2（7-9）。

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) (Alexa Fluor® 488 Conjugate) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody.
Protect from light. Do not freeze.