Phospho-Elk-1 (Ser383) Antibody

• 产品编号：CST-9181S      品牌：CST       原厂货号：9181S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Elk-1 (Ser383)抗体能检测少于5 ng的重组磷酸化Elk-1蛋白，同时也不与大于1 µg非磷酸化Elk-1蛋白反应。该多克隆抗体是通过用与人源Elk-1蛋白丝氨酸（383位点）周围残基相一致的磷酸化的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。转录因子Elk-1是一个三元复合物的组成部分，该复合物能结合血清效应因子(SRE)，调节基因活性以应答血清和生长因子(1-3)。MAP激酶能将Elk-1蛋白羧端的一串S/T基序磷酸化，这些位点的磷酸化，特别是丝氨酸（383位点）的磷酸化，对Elk-1蛋白的转录活性很重要。Elk-1蛋白似乎是活化的MAP激酶直接的靶点：(a)生化研究表明Elk-1蛋白是MAP激酶很好的底物；(b) Elk-1蛋白磷酸化和活化的动力学与MAP激酶活性有关；(c) MAP激酶的干扰突变体会阻碍体内Elk-1蛋白的活化。其他研究表明丝氨酸（383位点）磷酸化的Elk-1蛋白也是应激性活化激酶SAPK/JNK的靶点(4,5)。

1. Marais, R. et al. (1993) Cell 73, 381-393.
2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse, Rat, Monkey, Zebrafish, Dog

Specificity / Sensitivity

Phospho-Elk-1 (Ser383) Antibody detects less than 5 ng of phosphorylated Elk-1, and will not react with up to 1 µg of nonphosphorylated EIk-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

1. Marais, R. et al. (1993) Cell 73, 381-393.
2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.