PhosphoPlus? Elk-1 (Ser383) Antibody Kit

产品编号：CST-9180S 品牌：CST 原厂货号：9180S
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
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包装：

运保温度:

–20°C

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其它组分：

20X LumiGLO® Reagent and 20X Peroxide 【子货号：#7003，包装：5 ml each，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody【子货号： #7074，包装：100 µl，，运保温度：–20°C】

Anti-biotin, HRP-linked Antibody 【子货号：#7075，包装：100 µl，，运保温度：–20°C】

Biotinylated Protein Ladder Detection Pack 【子货号：#7727，包装：100 µl，，运保温度：–20°C】

Phospho-Elk-1 (Ser383) Antibody 【子货号：#9181，包装：100 µl，，运保温度：–20°C】

Elk-1 Antibody 【子货号：#9182，包装：100 µl，，运保温度：–20°C】

Elk-1 Control Proteins 【子货号：#9183，包装：150 µl，，运保温度：–20°C】

描述：

Elk-1兔多抗能检测内源性总Elk-1蛋白水平。Phospho-Elk-1 (Ser383) Antibody 兔多抗能检测丝氨酸（383位点）磷酸化的Elk-1蛋白。该多克隆抗体是通过用与人源Elk-1蛋白丝氨酸（383位点）周围残基相一致的磷酸化的合成肽段，或从人源Elk-1蛋白序列演变而来的合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。PhosphoPlus® Elk-1 (Ser383) Antibody Kit为快速分析Elk-1蛋白磷酸化位点提供试剂和说明书。在细菌上表达的Elk-1蛋白作为阴性对照。用p42 MAP Kinase (Erk2)磷酸化的同种蛋白作为阳性对照。转录因子Elk-1是一个三元复合物的组成部分，该复合物能结合血清效应因子(SRE)，调节基因活性以应答血清和生长因子(1-3)。MAP激酶能将Elk-1蛋白羧端的一串S/T基序磷酸化，这些位点的磷酸化，特别是丝氨酸（383位点）的磷酸化，对Elk-1蛋白的转录活性很重要。Elk-1蛋白似乎是活化的MAP激酶直接的靶点：(a)生化研究表明Elk-1蛋白是MAP激酶很好的底物；(b) Elk-1蛋白磷酸化和活化的动力学与MAP激酶活性有关；(c) MAP激酶的干扰突变体会阻碍体内Elk-1蛋白的活化。其他研究表明丝氨酸（383位点）磷酸化的Elk-1蛋白也是应激性活化激酶SAPK/JNK的靶点(4,5)。

原厂资料：

Specificity / Sensitivity

Elk-1 Antibody detects endogenous levels of total Elk-1 protein. Phospho-Elk-1 (Ser383) Antibody detects EIk-1 protein only when activated by phosphorylation at serine 383.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser383 of human Elk-1, or with a synthetic peptide derived from the sequence of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.

Description

The PhosphoPlus® Elk-1 (Ser383) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of Elk-1. Bacterially expressed Elk-1 serves as a negative control. The same protein phosphorylated by p42 MAP Kinase (Erk2) serves as a positive control.

Background

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).

注意事项：

Storage: Antibodies are supplied in 10 mM sodium HEPES
(pH 7.5), 150 mM NaCI, 100 μg/ml BSA and 50% glycerol.
Store at –20°C. Do not aliquot the antibodies