Species predicted to react based on 100% sequence homology:Xenopus, Zebrafish
Specificity / Sensitivity
Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody detects endogenous levels of SEK1/MKK4 phosphorylated at serine 257/threonine 261 . This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. It will also react with SEK1/MKK4 singly phosphorylated at Thr261.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).