Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb

• 产品编号：CST-9145S      品牌：CST       原厂货号：9145S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb只能检测内源的在tyr705位点磷酸的 Stat3 蛋白。此抗体不与磷酸化的EGFR和对应的磷酸化酪氨酸残基的其它Stat 蛋白反应。单克隆抗体是通过合成鼠源对应的Stat3 Tyr705位点周围的磷肽段来免疫动物而获得。Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1)，并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活，从而导致它的二聚化，入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能，Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型，配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
7. Ihle, J.N. (1995) Nature 377, 591-4.
8. Wen, Z. et al. (1995) Cell 82, 241-50.
9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Pig, Horse

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
7. Ihle, J.N. (1995) Nature 377, 591-4.
8. Wen, Z. et al. (1995) Cell 82, 241-50.
9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.