PhosphoPlus? Stat3 (Tyr705) Antibody Kit

• 产品编号：CST-9130S      品牌：CST       原厂货号：9130S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
• 应用分类：

其它组分：

描述：

Phospho-Stat3 (Tyr705) Antibody只能检测内源的在tyr705位点磷酸的 Stat3 蛋白。此抗体不与在对应酪氨酸残基磷酸化的其它Stat 蛋白交叉反应，但是与EGFR交叉反应。能够检测内源水平Stat3蛋白总体水平。Phospho-Stat3 (Tyr705)多克隆抗体是通过合成鼠源对应的Stat3 Tyr705位点周围的磷肽段来免疫动物而获得。Stat3多克隆抗体是通过合成鼠源Stat3对应的多肽免疫动物而获得。 抗体是通过protein A和多肽亲和层析法纯化。Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1)，并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活，从而导致它的二聚化，入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能，Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型，配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
7. Ihle, J.N. (1995) Nature 377, 591-4.
8. Wen, Z. et al. (1995) Cell 82, 241-50.
9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

原厂资料：

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue, but does cross-react with EGFR. Stat3 antibody detects endogenous levels of total Stat3 protein.

Source / Purification

Polyclonal antibodies to Phospho-Stat3 are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3 (Phospho-Stat3 (Tyr705) Antibody), or with a synthetic peptide corresponding to mouse Stat3 (Stat3 antibody). Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
7. Ihle, J.N. (1995) Nature 377, 591-4.
8. Wen, Z. et al. (1995) Cell 82, 241-50.
9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies