Phospho-MEK1 (Thr286) Antibody

• 产品编号：CST-9127S      品牌：CST       原厂货号：9127S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MEK1 (Thr286) Antibody兔多抗能够检测内源性苏氨酸（286位点）磷酸化的MEK1蛋白水平。该抗体不会与磷酸化的MEK2蛋白发生交叉反应。该多克隆抗体是采用合成的人源MEK1蛋白苏氨酸（286位点）周围相一致的磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。MEK1的Ser298位点被PAK1磷酸化后，能够促进信号从Raf 到 MEK1、Erk2的转导(5-7)。在处于有丝分裂的细胞中，MEK1的Thr286位点也会被cdk5磷酸化，导致p44/42 MAP激酶途径的负反馈 (8)。

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.
5. Xu, B. et al. (1999) J. Biol. Chem. 274, 34029-34035.
6. Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-2244.
7. Eblen, S. T. et al. (2002) Mol. Cell. Biol. 22, 6023-6033.
8. Sharma, P. et al. (2002) J. Biol. Chem. 277, 528-534.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-MEK1 (Thr286) Antibody detects endogenous levels of MEK1 phosphorylated at threonine 286. This antibody does not cross-react with phosphorylated MEK2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human MEK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8).

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.
5. Xu, B. et al. (1999) J. Biol. Chem. 274, 34029-34035.
6. Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-2244.
7. Eblen, S. T. et al. (2002) Mol. Cell. Biol. 22, 6023-6033.
8. Sharma, P. et al. (2002) J. Biol. Chem. 277, 528-534.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody