Phospho-MEK1/2 (Ser217/221) Antibody

• 产品编号：CST-9121S      品牌：CST       原厂货号：9121S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MEK1/2 (Ser217/221) Antibody兔多抗能够检测内源性的Ser217/221位点磷酸化的MEK1/2蛋白水平。该抗体不会与其他相关激酶包括活化的SEK (MKK4), MKK3、MKK6发生交叉反应。它也能与Ser217位点和Ser221位点单独磷酸化的MEK1/2蛋白发生反应。该单克隆抗体是采用合成的与人源 MEK1/2蛋白Ser217/221位点周围相一致的磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。CST的 Phospho- MEK1/2 (Ser217/221) Antibody兔多抗能选择性的识别活化的的MEK，即Ser217/221位点磷酸化的MEK，因此它是表征MEK1/2活性的重要标志。

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. This antibody does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.CST's Phospho- MEK1/2 (Ser217/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.

1. Crews, C.M. et al. (1992) Science 258, 478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
4. Cowley, S. et al. (1994) Cell 77, 841-852.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody