PhosphoPlus? MEK1/2 (Ser217/221) Antibody Kit

产品编号：CST-9120S 品牌：CST 原厂货号：9120S
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
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包装：

运保温度:

-20°C.

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其它组分：

20X LumiGLO® Reagent and 20X Peroxide 【子货号：#7003，包装：5 ml each，，运保温度：-20°C.】

Anti-rabbit IgG, HRP-linked Antibody【子货号： #7074，包装：100 µl，，运保温度：-20°C.】

Anti-biotin, HRP-linked Antibody 【子货号：#7075，包装：100 µl，，运保温度：-20°C.】

Biotinylated Protein Ladder Detection Pack 【子货号：#7727，包装：100 µl，，运保温度：-20°C.】

MEK1/2 (D1A5) Rabbit mAb 【子货号：#8727，包装：100 µl，，运保温度：-20°C.】

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb 【子货号：#9154，包装：100 µl，，运保温度：-20°C.】

MEK1/2 HeLa Control Cell Extracts 【子货号：#9160，包装：200 µl，，运保温度：-20°C.】

描述：

当与Phototope®-HRP Western Detection System联合使用时，Phospho-MEK1/2 (Ser217/221) Antibody兔多抗对磷酸化的MEK1的检测灵敏度可达1ng,对非磷酸化的MEK1在多达1ug时也不会产生非特异结合。Phospho-MEK1/2 (Ser217/221) Antibody兔多抗能检测内源性Ser217、221位点双磷酸化的MEK1/2蛋白水平，也能与Ser221位点和Ser217位点分别单磷酸化的MEK1/2蛋白反应。MEK1/2 antibody兔多抗能够检测内源性MEK1/2总蛋白水平。任何一个抗体都不会与相关激酶发生交叉反应，如SEK1 (MKK4), MKK3和MKK6。该单克隆抗体是采用合成的与人源 MEK1/2蛋白Ser217/221位点周围序列相一致的磷酸化肽段免疫动物而产生的。该多克隆抗体是采用合成的与人源 MEK1/2蛋白序列相一致的合成肽段免疫动物而产生的。PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit为快速检测Ser217/221位点磷酸化的MEK1/2蛋白提供了试剂及方案。MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。

原厂资料：

Specificity / Sensitivity

When used in conjunction with our Phototope®-HRP Western Detection System, Phospho-MEK1/2 (Ser217/221) Antibody detects 1 ng phosphorylated MEK1/2 yet will not react with up to 1 µg of nonphosphorylated MEK1/2.     Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at serines 217 and 221. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221. MEK1/2 Antibody detects endogenous levels of total MEK1/2 protein. Neither antibody cross-reacts with related kinases, such as SEK1 (MKK4), MKK3 or MKK6.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues corresponding to the sequence of human MEK1/2 (MEK1/2 Antibody).

Description

The PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of MEK1/2 at Ser217/221.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

注意事项：

Storage: Antibodies are supplied in 10 mM sodium HEPES
(pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol.
Store at -20°C. Do not aliquot the antibodies.