MARK2 Antibody

• 产品编号：CST-9118S      品牌：CST       原厂货号：9118S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MARK2 抗体识别内源性的MARK2总蛋白。没有观察到与其它MARK家族成员发生交叉反应。通过合成与人源MARK2蛋白Lys430邻近氨基酸序列一致的肽段，免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。微管相关蛋白调节微管的稳定性和控制相关进程例如细胞极化/分化、轴突生长、细胞分裂和细胞器的运输(1)。丝氨酸/苏氨酸激酶的MARK (MAP/microtubule affinity-regulating kinases)家族(MARK1-4)被鉴定基于它们的有能力去磷酸化microtubule-associated proteins (MAPs)包括tau、MAP2和MAP4蛋白(2-6)。MARK蛋白在它们的微管结合区域里磷酸化MAPs，这就引起MAPs蛋白从微管上分离，并且增加了微管的动力(2-4)。至于tau蛋白，在阿尔茨海默病人中观察其磷酸化已经被假设有助于神经纤维缠结的形成。MARK激酶的过表达导致MAPs的过磷酸化 、形态学改变和细胞死亡(4)。肿瘤抑制激酶LKB1磷酸化MARK激酶和紧密相关的含有T-loops的AMP-kinases ，导致活性的增加(7)。MARK2 (4)又称为Par-1 (8)和EMK1 (9)，其有助于细胞极化、细胞周期进程、微管动力和轴突生长。MARK2基因编码至少两个选择性剪切形式，它们可在不同的细胞中共表达(10)。MARK2基因的底物包括微管相关蛋白(MAPs)、tau、组蛋白去乙酰化酶(11)和Rab11-FIP2蛋白(12)。基因敲除研究认为MARK2激酶在免疫系统功能 (13)、血糖稳定(14)和学习记忆(15)中起着本质作用。

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
8. Guo, S. and Kemphues, K.J. (1995) Cell 81, 611-620.
9. Inglis, J.D. et al. (1993) Mamm. Genome 4, 401-403.
10. Espinosa, L. and Navarro, E. (1998) Cytogenet. Cell Genet. 81, 278-282.
11. Dequiedt, F. et al. (2006) Mol. Cell. Biol. 26, 7086-7102.
12. Ducharme, N.A. et al. (2006) Mol. Biol. Cell 17, 3625-3637.
13. Hurov, J.B. et al. (2001) Mol. Cell. Biol. 21, 3206-3219.
14. Hurov, J.B. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 5680-5685.
15. Segu, L. et al. (2008) Neurobiol. Aging 29, 231-240.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

MARK2 Antibody detects endogenous levels of total MARK2 protein. No cross reactivity is observed with other MARK family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys430 of human MARK2. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).MARK2 (4), also termed as Par-1 (8) and EMK1 (9), contributes to cellular polarity, cell cycle progression, microtuble dynamics, and neurite outgrowth. The MARK2 gene encodes at least two alternatively spliced isoforms that are co-expressed in various cell lines (10). Substrates of MARK2 include microtubule associated protein (MAPs), tau, histone deacetylases (11), and Rab11-FIP2 (12). Knockout studies suggest that MARK2 plays an essential role in immune system function (13), glucose homeostasis (14), and learning and memory (15).

1. Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
2. Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
3. Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
4. Drewes, G. et al. (1997) Cell 89, 297-308.
5. Kato, T. et al. (2001) Neoplasia 3, 4-9.
6. Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
7. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
8. Guo, S. and Kemphues, K.J. (1995) Cell 81, 611-620.
9. Inglis, J.D. et al. (1993) Mamm. Genome 4, 401-403.
10. Espinosa, L. and Navarro, E. (1998) Cytogenet. Cell Genet. 81, 278-282.
11. Dequiedt, F. et al. (2006) Mol. Cell. Biol. 26, 7086-7102.
12. Ducharme, N.A. et al. (2006) Mol. Biol. Cell 17, 3625-3637.
13. Hurov, J.B. et al. (2001) Mol. Cell. Biol. 21, 3206-3219.
14. Hurov, J.B. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 5680-5685.
15. Segu, L. et al. (2008) Neurobiol. Aging 29, 231-240.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.