Maspin (T50) Antibody

• 产品编号：CST-9117S      品牌：CST       原厂货号：9117S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Maspin (T50) Antibody 能够检测内源水平maspin总蛋白。多克隆抗体是采用合成人 maspin Thr50周围残基相对应的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Maspin (SERPINB5)是乳腺肿瘤的抑制因子，它在正常的乳腺上皮中表达，但是在很多乳腺癌细胞系中缺失(1)。Maspin 与 serpin 家族的 serine 蛋白酶抑制剂有关联, 它可能不是作为蛋白酶抑制剂而发挥作用(2)。它通过在细胞粘连中发挥重要的作用，而在胚胎发育过程中起重要作用(3)。Maspin信号通路的具体机制尚不清楚(4)，其具有启动凋亡能力（8），其肿瘤抑制活性是由于它有抑制细胞入侵/转移(5,6) 和血管再生的能力(7)。活性IKKα的核定位能够抑制maspin的转录，并促进前列腺癌的转移(9)。

1. Zou, Z. et al. (1994) Science 263, 526-9.
2. Pemberton, P.A. et al. (1995) J Biol Chem 270, 15832-7.
3. Gao, F. et al. (2004) Development 131, 1479-89.
4. Bailey, C.M. et al. (2006) J Cell Physiol 209, 617-24.
5. Sheng, S. et al. (1996) Proc Natl Acad Sci USA 93, 11669-74.
6. Seftor, R.E. et al. (1998) Cancer Res 58, 5681-5.
7. Zhang, M. et al. (2000) Nat Med 6, 196-9.
8. Jiang, N. et al. (2002) Oncogene 21, 4089-98.
9. Luo, J.L. et al. (2007) Nature 446, 690-4.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Maspin (T50) Antibody detects endogenous levels of total maspin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human maspin. Antibodies were purified by peptide affinity chromatography.

Background

Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9).

1. Zou, Z. et al. (1994) Science 263, 526-9.
2. Pemberton, P.A. et al. (1995) J Biol Chem 270, 15832-7.
3. Gao, F. et al. (2004) Development 131, 1479-89.
4. Bailey, C.M. et al. (2006) J Cell Physiol 209, 617-24.
5. Sheng, S. et al. (1996) Proc Natl Acad Sci USA 93, 11669-74.
6. Seftor, R.E. et al. (1998) Cancer Res 58, 5681-5.
7. Zhang, M. et al. (2000) Nat Med 6, 196-9.
8. Jiang, N. et al. (2002) Oncogene 21, 4089-98.
9. Luo, J.L. et al. (2007) Nature 446, 690-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody