Phospho-cdc2 (Tyr15) Antibody

• 产品编号：CST-9111S      品牌：CST       原厂货号：9111S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-cdc2 (Tyr15) Antibody 能够检测内源性酪氨酸（15位）磷酸化的cdc2, CDK2 和CDK5蛋白。该抗体不与CDK4, CDK6或CDK7发生交叉反应，但能够识别酪氨酸19位磷酸化cdc2 (cdc28)蛋白的酵母同源序列。该多克隆抗体是由合成的人源的针对cdc2蛋白酪氨酸(15位)磷酸化肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。

1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
5. Hunter, T. (1995) Cell 80, 225-36.

原厂资料：

Specificity / Sensitivity

Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. The antibody does not cross-react with CDK4, CDK6 or CDK7. It does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
5. Hunter, T. (1995) Cell 80, 225-36.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.