DC-SIGN Antibody

• 产品编号：CST-9097S      品牌：Cell Signaling Technology       原厂货号：9097S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DC-SIGN Antibody能识别内源性DC-SIGN总蛋白水平。此抗体也能检测DC-SIGNR。多克隆抗体通过合成肽免疫动物制备，该合成肽是人DC-SIGN蛋白Val134周围的残基。抗体通过protein A以及多肽亲和色谱法纯化。DC-SIGN (CD209/CLEC4L)是一种C型凝集素受体，由树突状细胞(DCs)表达(1,2)。DC-SIGN转录本能经受数次剪接从而产生至少13种不同的跨膜和可溶性亚型（3）。由于DC-SIGN能同时识别甘露糖和果糖碳水化合物，因此它对很多病原体都有应答，并且它在HIV感染中的作用已经研究得比较明确。DC-SIGN对HIV包膜糖蛋白gp120的识别引起DCs对HIV的细胞内摄作用和促进病毒向CD4⁺ T细胞的传输（2,4）。DC-SIGN还通过与ICAM-3相互作用介导对T细胞的黏附和通过与ICAM-2结合穿过内皮（1,5）。DC-SIGN能通过激活激酶Raf-1来调解TLR信号（6,7）。DC-SIGNR (L-SIGN/CLEC4M)是一种密切相关分子，与DC-SIGN有77%的同源性，可能通过基因复制出现（8）。和DC-SIGN一样，DC-SIGNR也能与病原体表面的甘露糖碳水化合物结合（8,9）。然而，两种受体的表达模式并不一样，DC-SIGNR的表达局限于肝脏内皮细胞、淋巴结和胎盘（10）。在小鼠中，有一系列相关分子，SIGNR1-SIGNR8 (11)。基于序列分析发现其与人DC-SIGN并不具有同源性，尽管SIGNR3也能同时识别甘露糖和果糖结构（11）。

1. Geijtenbeek, T.B. et al. (2000) Cell 100, 575-85.
2. Geijtenbeek, T.B. et al. (2000) Cell 100, 587-97.
3. Mummidi, S. et al. (2001) J Biol Chem 276, 33196-212.
4. Kwon, D.S. et al. (2002) Immunity 16, 135-44.
5. Geijtenbeek, T.B. et al. (2000) Nat Immunol 1, 353-7.
6. Gringhuis, S.I. et al. (2007) Immunity 26, 605-16.
7. Gringhuis, S.I. et al. (2010) Nat Immunol 11, 419-26.
8. Bashirova, A.A. et al. (2001) J Exp Med 193, 671-8.
9. Mitchell, D.A. et al. (2001) J Biol Chem 276, 28939-45.
10. Pöhlmann, S. et al. (2001) Proc Natl Acad Sci U S A 98, 2670-5.
11. Powlesland, A.S. et al. (2006) J Biol Chem 281, 20440-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

DC-SIGN Antibody recognizes endogenous levels of total DC-SIGN protein. This antibody also detects DC-SIGNR. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val134 of human DC-SIGN protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DC-SIGN (CD209/CLEC4L) is a C-type lectin receptor expressed by dendritic cells (DCs) (1,2). The DC-SIGN transcript can undergo several splicing events to generate at least thirteen different transmembrane and soluble isoforms (3). DC-SIGN responds to a broad range of pathogens due to its ability to recognize both mannose and fructose carbohydrates, and is well-studied for its role in HIV infection. Recognition of the HIV envelope glycoprotein gp120 by DC-SIGN leads to internalization of HIV by DCs and facilitates transmission of the virus to CD4+ T cells (2,4). DC-SIGN also mediates adhesion to T cells through interaction with ICAM-3, as well as transmigration across the endothelium by binding to ICAM-2 (1,5). DC-SIGN can also modulate TLR signaling by activating the kinase Raf-1 (6,7). DC-SIGNR (L-SIGN/CLEC4M) is a closely related molecule that is 77% homologous to DC-SIGN and likely arose through a gene duplication event (8). Like DC-SIGN, DC-SIGNR binds mannose carbohydrates on the surface of pathogens (8,9). However, the expression patterns of the two receptors differ, as DC-SIGNR expression is restricted to endothelial cells of the liver, lymph node, and placenta (10). In mice, there is a set of related molecules, SIGNR1-SIGNR8 (11). Based on sequence analysis, there is no clear ortholog to human DC-SIGN, however SIGNR3 is the most functionally similar due to its ability to recognize both mannose and fructose structures (11).

1. Geijtenbeek, T.B. et al. (2000) Cell 100, 575-85.
2. Geijtenbeek, T.B. et al. (2000) Cell 100, 587-97.
3. Mummidi, S. et al. (2001) J Biol Chem 276, 33196-212.
4. Kwon, D.S. et al. (2002) Immunity 16, 135-44.
5. Geijtenbeek, T.B. et al. (2000) Nat Immunol 1, 353-7.
6. Gringhuis, S.I. et al. (2007) Immunity 26, 605-16.
7. Gringhuis, S.I. et al. (2010) Nat Immunol 11, 419-26.
8. Bashirova, A.A. et al. (2001) J Exp Med 193, 671-8.
9. Mitchell, D.A. et al. (2001) J Biol Chem 276, 28939-45.
10. Pöhlmann, S. et al. (2001) Proc Natl Acad Sci U S A 98, 2670-5.
11. Powlesland, A.S. et al. (2006) J Biol Chem 281, 20440-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.