StemLight? Pluripotency Transcription Factor Antibody Kit

• 产品编号：CST-9093S      品牌：Cell Signaling Technology       原厂货号：9093S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Nanog (D73G4) XP^® Rabbit mAb能够检测内源性的总Nanog蛋白。Oct-4A (C30A3) Rabbit mAb能够识别内源性的总Oct-4A蛋白。Sox2 (D6D9) XP^® Rabbit mAb能够识别内源性的总Sox2蛋白。合成与邻近人源Nanog蛋白的氨基末端，与人源Oct-4A的重组蛋白、与人源Sox2蛋白的Gly179氨基酸残基序列一致的肽段，免疫动物获得单克隆抗体。StemLight多潜能性转录因子抗体试剂盒包括一系列能够检测ES和iPS细胞中主要转录网络中关键组分蛋白包括Oct-4, Nanog, 和Sox2的抗体。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。试剂盒中组分已经优化为标准稀释度用于在免疫荧光分析中配套使用，但是这些组件也能用于其他实验——请参阅单独的使用说明以寻找具体建议。试剂足够进行160次反应体系为100ul的免疫荧光实验。具有分化潜能的细胞能够分化为三种胚层，包括内胚层，外胚层和中胚层细胞。胚胎干细胞，胚胎瘤细胞和诱导多能干细胞均具有多潜能性。Oct-4, Sox2 和Nanog是在多潜能干细胞中高表达的重要转录因子（1）。它们共同构成转录调节网络，保持细胞处于多潜能状态（2,3）。过表达Oct-4 和Sox2以及Klf4和 c- Myc能诱导小鼠和人的体细胞成为具有多潜能性的细胞，说明在维持细胞更新和多潜能性所需的转录网络中它们是关键的调控因子（4-5）。过表达Oct-4 ,Sox2,Nanog和Lin28也能赋予人体细胞具备多潜能性（6）。在多潜能干细胞分化过程中，Oct-4 ,Sox2和Nanog表达量下降。

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
5. Okita, K. et al. (2007) Nature 448, 313-7.
6. Yu, J. et al. (2007) Science 318, 1917-20.

原厂资料：

Specificity / Sensitivity

Nanog (D73G4) XP^® Rabbit mAb detects endogenous levels of total Nanog protein. Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP^® Rabbit mAb detects endogenous levels of total Sox2 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Nanog protein, recombinant protein corresponding to the amino terminus of human Oct-4A, and a synthetic peptide corresponding to residues surrounding Gly179 of human Sox2.

Description

StemLight™ Pluripotency Transcription Factor Antibody Kit contains a panel of antibodies for the detection of Oct-4, Nanog, and Sox2, key components of the core pluripotency transcription network in embryonic stem (ES) and induced pluripotent stem (iPS) cells. The kit can be used to track the pluripotent potential of human ES or iPS cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications - please refer to individual datasheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.

Background

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells. 
 Oct-4, Sox2, and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2, along with KLF4 and c-Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcriptional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog, and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog, and Sox2 is downregulated.

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
5. Okita, K. et al. (2007) Nature 448, 313-7.
6. Yu, J. et al. (2007) Science 318, 1917-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies