eIF2α (D7D3) XP? Rabbit mAb (Biotinylated)

• 产品编号：CST-9079S      品牌：CST       原厂货号：9079S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

eIF2α (D7D3) XP® Rabbit mAb (Biotinylated) 能够检测内源性eIF2α总蛋白水平。该单克隆抗体是经由纯化的人eIF2α重组蛋白免疫动物而生产的。该CST抗体在最优条件下偶联了生物素。该生物素化抗体与未接合的 eIF2α (D7D3) XP® Rabbit mAb兔单抗 #5324具有相同的种属交叉反应性。研究证明在不同的应激条件下真核生物起始因子2 (eIF2) α亚型的磷酸化可下调蛋白质的合成。eIF2蛋白与GTP以及起始tRNA（Met-tRNAi）结合，然后将Met-tRNA转移到40S核糖体亚基上共同形成43S前起始复合物(1,2)。eIF2通过eIF2B催化反应将GTP交换GDP从而促进新一轮翻译的起始(1,2)。病毒感染(PKR)、内质网应激（endoplasmic reticulum stress）(PERK/PEK)、氨基酸饥饿(GCN2)或血红素缺乏症(HRI)能够激活相关的激酶，这些激酶可以使eIF2的α亚基磷酸化(3,4)。这个磷酸化稳固eIF2-GDP-eIF2B复合物，同时期抑制eIF2B的降解。通过IFN-γ 和TNF-α的诱导的PKR能有效的诱导eIF2α蛋白在Ser51位点磷酸化(5,6)。

1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

原厂资料：

Specificity / Sensitivity

eIF2α (D7D3) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total eIF2α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a purified recombinant human eIF2α protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated eIF2α (D7D3) XP® Rabbit mAb #5324.

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.