MitoTracker^? Green FM

• 产品编号：CST-9074S      品牌：CST       原厂货号：9074S
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞结构探针 > 基于荧光染料的细胞结构探针
• 应用分类：

描述：

每小瓶MitoTracker® Green FM包含50 μg冻干的固体。对于1 mM浓度贮存液，在冻干粉中加入74.4 μl 高品质的DMSO。最佳染色浓度和孵育时间根据应用的不同而有所不同。将贮存液直接用普通生长培养基稀释到浓度为100-400 nM，37ºC培养15-30分钟后，可获得典型的结果。培养过后，细胞必须实时成像，不要固定！为了减少培养基的背景荧光，在成像前用没有酚红的培养基代替普通培养基。绿色背景抑制剂，如Life Technologies公司的BackDrop™ Background Suppressor，也可以用来减少背景荧光。MitoTracker® dyes是一种细胞可透过的探针，包含一个轻度巯基活性的氯甲基部分来进行线粒体的标记。为了标记线粒体，只需将细胞与MitoTracker® probes一起培养，然后探针就会被动扩散，穿过质膜，在有活性的线粒体中积聚。

原厂资料：

Directions for Use

Each vial of MitoTracker® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX! 
 
 To reduce background fluorescence of the media, phenol-red free media can be substituted for normal media prior to imaging. A green background suppressor, such as BackDrop™ Background Suppressor from Life Technologies, can also be added to reduce background fluorescence.

Background

MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

注意事项：

Storage: Store lyophilized solid at -20ºC dessicated and
protected from light. In lyophilized form, this reagent is stable for
6 months. Once reconstituted in DMSO, the solution should be
stored at -20ºC, protected from light, and must be used within 2
weeks. Avoid freeze-thaw cycles.