Dectin-1 Antibody

• 产品编号：CST-9051S      品牌：Cell Signaling Technology       原厂货号：9051S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dectin-1 抗体能识别内源性Dectin-1总蛋白水平。此抗体与来源不明的80kDa蛋白有交叉反应。Dectin-1转录物的选择性剪接产生了八个亚型，预期的分子量为5-27kDa。糖基化使得Dectin-1的分子量比预期的大。多克隆抗体通过合成肽免疫动物制备，该合成肽是人Dectin-1蛋白氨基端附近的残基。抗体通过蛋白A以及多肽亲和色谱法纯化。Dectin-1是C型凝集素受体，在巨噬细胞、单核细胞、树突细胞、中性白细胞和γδ T细胞亚型都能表达（1,2）。Dectin-1是一种糖蛋白，由于选择性剪接有八个不同的亚型（3-5）。Dectin-1通过作为一种模式识别受体，识别真菌和一些细菌细胞壁上的β葡聚糖类，从而在抗真菌免疫中起到了重要作用（5,6）。Dectin-1由一个短的氨基端胞浆区（包含一个ITAM样基序）、一个跨膜区和一个细胞外羧基端C型凝集素结构域组成（5）。Dectin-1能通过其C型凝集素结构域识别β葡聚糖类，通过其ITAM样基序募集和激活Syk识别转导信号（7,8）。Dectin-1通过产生IL-6和IL-23促进Th17细胞的分化来激活树突状细胞（9）。

1. Taylor, P.R. et al. (2002) J Immunol 169, 3876-82.
2. Martin, B. et al. (2009) Immunity 31, 321-30.
3. Ariizumi, K. et al. (2000) J Biol Chem 275, 20157-67.
4. Kato, Y. et al. (2006) Biol Pharm Bull 29, 1580-6.
5. Willment, J.A. et al. (2001) J Biol Chem 276, 43818-23.
6. Brown, G.D. and Gordon, S. (2001) Nature 413, 36-7.
7. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
8. Underhill, D.M. et al. (2005) Blood 106, 2543-50.
9. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Dectin-1 Antibody recognizes endogenous levels of total Dectin-1 protein. This antibody cross-reacts with an 80 kDa protein of unknown origin. Alternative splicing of the Dectin-1 transcript can lead to eight isoforms with predicted molecular weights ranging from 5-27 kDa. Glycosylation causes Dectin-1 to run higher than its predicted molecular weight.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Dectin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Dectin-1 is a C-type lectin receptor expressed by macrophages, monocytes, dendrtic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).

1. Taylor, P.R. et al. (2002) J Immunol 169, 3876-82.
2. Martin, B. et al. (2009) Immunity 31, 321-30.
3. Ariizumi, K. et al. (2000) J Biol Chem 275, 20157-67.
4. Kato, Y. et al. (2006) Biol Pharm Bull 29, 1580-6.
5. Willment, J.A. et al. (2001) J Biol Chem 276, 43818-23.
6. Brown, G.D. and Gordon, S. (2001) Nature 413, 36-7.
7. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
8. Underhill, D.M. et al. (2005) Blood 106, 2543-50.
9. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody