Miz-1 Antibody

• 产品编号：CST-9044S      品牌：Cell Signaling Technology       原厂货号：9044S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Miz-1抗体能识别内源性水平的总Miz-1蛋白。此抗体在某些细胞系中与来源不明的60kDa蛋白有交叉反应。多克隆抗体通过合成肽免疫动物制备，该合成肽是人Miz-1蛋白Pro158周围的残基。抗体通过蛋白A以及多肽亲和色谱法纯化。Miz-1 (Zbtb17)是一种痘病毒和锌指(POZ)转录因子，有一个氨基端的BTB/POZ结构域和13个羧基端的锌指结构域。Miz-1通过CDK抑制蛋白(p15，INK4B和p21 Waf1/Cip1)的活化在细胞周期控制中有重要作用（1-4）。与Myc的直接相互作用能抑制Miz-1的转录活性（1-4）。在DNA损伤中，Myc通过Miz-1募集到p21 Waf1/Cip1启动子上，并阻止p53介导的p21 Waf1/Cip1的诱导表达，最终引起p53介导的细胞凋亡而不是细胞周期停滞（4）。Miz-1在淋巴细胞发育过程中也发挥作用。在B和T细胞发育中，Miz-1抑制细胞因子信号抑制蛋白1(SOCS1)的表达，使信号穿过IL-7受体，并使促存活蛋白Bcl-2表达上调（5,6）。

1. Peukert, K. et al. (1997) EMBO J 16, 5672-86.
2. Seoane, J. et al. (2001) Nat Cell Biol 3, 400-8.
3. Staller, P. et al. (2001) Nat Cell Biol 3, 392-9.
4. Seoane, J. et al. (2002) Nature 419, 729-34.
5. Kosan, C. et al. (2010) Immunity 33, 917-28.
6. Saba, I. et al. (2011) Blood 117, 3370-81.

原厂资料：

Specificity / Sensitivity

Miz-1 Antibody recognizes endogenous levels of total Miz-1 protein. This antibody cross-reacts with a 60 kDa protein of unknown origin in some cell lines.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro158 of human Miz-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Miz-1 (Zbtb17) is a poxvirus and zinc finger (POZ) transcription factor with an amino-terminal BTB/POZ domain and 13 carboxy-terminal zinc finger domains. Miz-1 plays a key role in cell cycle control through activation of the cyclin-dependent kinase inhibitors p15, INK4B, and p21 Waf1/Cip1 (1-4). The transcriptional activity of Miz-1 is repressed through direct interaction with Myc (1-4). In the presence of DNA damage, Myc is recruited to the p21 Waf1/Cip1 promoter by Miz-1 and blocks p53-mediated induction of p21 Waf1/Cip1, ultimately resulting in p53-mediated apoptosis rather than cell cycle arrest (4). Miz-1 also plays a role during lymphocyte development. In developing B and T cells, Miz-1 represses suppressor of cytokine signaling 1 (SOCS1) expression, which enables signaling through the IL-7 receptor and upregulation of the pro-survival protein Bcl-2 (5,6).

1. Peukert, K. et al. (1997) EMBO J 16, 5672-86.
2. Seoane, J. et al. (2001) Nat Cell Biol 3, 400-8.
3. Staller, P. et al. (2001) Nat Cell Biol 3, 392-9.
4. Seoane, J. et al. (2002) Nature 419, 729-34.
5. Kosan, C. et al. (2010) Immunity 33, 917-28.
6. Saba, I. et al. (2011) Blood 117, 3370-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody