MAG (D4G3) XP? Rabbit mAb

• 产品编号：CST-9043P      品牌：Cell Signaling Technology       原厂货号：9043P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MAG (D4G3) XP® Rabbit mAb兔单抗能够检测内源性的MAG总蛋白。该单克隆抗体是由合成的人源MAG蛋白精氨酸(605位)肽段免疫动物而制备的。髓鞘相关糖蛋白（MAG），包含5个免疫球蛋白样结构域，是一个高度糖基化的蛋白（1）。MAG是节间髓鞘的一个组成部分，具有多种功能。它们在中枢神经系统（CNS）是由少突胶质细胞形成，而在周围神经系统（PNS）是由雪旺氏细胞形成（2）。一个已知的MAG功能是其抑制损伤后的轴突再生。从成人背根神经节以及后小脑，视网膜，脊髓，海马和上颈上神经节神经元，它均能够抑制其轴突生长（3）。MAG与最内层髓鞘上的其它分子和近轴突表面的补充受体间的相互作用对于轴突的长期稳定是十分必要的。在没有MAG的情况下，髓磷酯依然会表达，但可以检测到长期的轴突变性和轴突骨架结构改变（4）。

1. Li, M. et al. (1996) J Neurosci Res 46, 404-14.
2. Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
3. Yamashita, T. et al. (2002) J Cell Biol 157, 565-70.
4. Mehta, N.R. et al. (2010) ACS Chem Neurosci 1, 215-222.

原厂资料：

Specificity / Sensitivity

MAG (D4G3) XP® Rabbit mAb recognizes endogenous levels of total MAG protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg605 of human MAG protein.

Background

Myelin-associated glycoprotein (MAG), which contains five immunoglobulin-like domains, is a highly glycosylated protein (1). MAG is a component of all myelinated internodes, whether formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS) (2), and has several functions. A known function of MAG is its inhibition of axonal regeneration after injury. It inhibits axonal outgrowth from adult dorsal root ganglion and in postnatal cerebellar, retinal, spinal, hippocampal, and superior cervical ganglion neurons (3). Interaction between MAG and several other molecules on the innermost wrap of myelin and complementary receptors on the opposing axon surface are required for long-term axon stability. Without MAG, myelin is still expressed, but long-term axon degeneration and altered axon cytoskeleton structure can be seen (4).

1. Li, M. et al. (1996) J Neurosci Res 46, 404-14.
2. Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
3. Yamashita, T. et al. (2002) J Cell Biol 157, 565-70.
4. Mehta, N.R. et al. (2010) ACS Chem Neurosci 1, 215-222.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody