This kit is intended to be used with cultured cell lines that are either adherent or in suspension. The Cell Fractionation Kit allows for the determination of the subcellular localization of proteins through the separation into three distinct fractions. Efficiency of separation can be measured using Cell Signaling Technology's Cell Fractionation Antibody Sampler Kit #11843.本试剂盒用于贴壁或悬浮培养的细胞系。
Description
The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.
Background
Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
注意事项：
Storage: Upon receipt, Protease Inhibitor Cocktail 5871 should
be stored at 4°C. All other components should be stored at
-20°C
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