Fragilis Antibody

• 产品编号：CST-9037S      品牌：Cell Signaling Technology       原厂货号：9037S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fragilis抗体可以识别内源性总的Fragilis蛋白。多抗由合成肽段免疫动物产生，该肽段与小鼠fragilis蛋白邻近Gln16氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Fragilis (Ifitm3)属于干扰素（IFN）-诱导跨膜蛋白家族（1），包括了5个高度相似并在哺乳动物中保守的跨膜蛋白（2）。被认为是小鼠胚胎发育过程中首个被发现用于标记生殖细胞能力起始的基因（3）。表达高水平fragilis蛋白的细胞也表达stella，一个只表达于谱系限制的生殖细胞（3）。对fragilis家族的研究可以有助于解释干扰素在胚胎发育时期发挥作用的机制（2）。

1. Deblandre, G.A. et al. (1995) J Biol Chem 270, 23860-6.
2. Lange, U.C. et al. (2003) BMC Dev Biol 3, 1.
3. Saitou, M. et al. (2002) Nature 418, 293-300.

原厂资料：

Specificity / Sensitivity

Fragilis Antibody recognizes endogenous levels of total fragilis protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln16 of mouse fragilis protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fragilis (Ifitm3) belongs to the interferon (IFN)-inducible transmembrane protein family (1), which consists of five highly similar transmembrane proteins that are conserved among mammalian species (2). It is thought to be the first gene to mark the onset of germ cell competence during embryonic development in mice (3). Cells expressing high levels of fragilis protein go on to express stella, a protein found exclusively in lineage-restricted germ cells (3). Studies on fragilis family members could help elucidate the mechanisms through which interferons act as signaling molecules during embryonic development (2).

1. Deblandre, G.A. et al. (1995) J Biol Chem 270, 23860-6.
2. Lange, U.C. et al. (2003) BMC Dev Biol 3, 1.
3. Saitou, M. et al. (2002) Nature 418, 293-300.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody