SPT5 Antibody

• 产品编号：CST-9033S      品牌：CST       原厂货号：9033S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SPT5 Antibody能够检测内源性SPT5总蛋白水平。该多克隆抗体是采用合成的与人源SPT5蛋白Gly803残基周围序列相对应的肽段免疫动物而生产的。通过蛋白A和多肽亲和层析纯化抗体获得。DRB-sensitivity inducing factor (DSIF)是有SPT4和SPT5组成的异源二聚体，它有能力去帮助和抑制RNA polymerase II (RNAPII)活性(1-3)。DSIF与NELF (Negative Elongation Factor)一起抑制RNAPII延伸，这导致RNAPII蛋白启动子近端暂停（proximal pausing），因为它等待别的信号去重新转录(4)。启动子近端暂停（proximal pausing）的释放是通过positive transcription elongation factor (P-TEFb)使RNAPII C-terminal domain (CTD)和NELF的磷酸化而发出的 (5)。 P-TEFb也使SPT5蛋白Thr4位点磷酸化，该位点存在于进化上保守的heptapeptide repeat结构域中。该磷酸化过程可以转变DSIF从一个转录抑制因子到一个激活因子，而激活形式对于转录延伸起着重要作用(6,7)。

1. Wada, T. et al. (1998) Genes Dev 12, 343-56.
2. Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67.
3. Gomes, N.P. et al. (2006) Genes Dev 20, 601-12.
4. Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
5. Buratowski, S. (2009) Mol Cell 36, 541-6.
6. Wada, T. et al. (1998) EMBO J 17, 7395-403.
7. Yamada, T. et al. (2006) Mol Cell 21, 227-37.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Guinea Pig, Horse

Specificity / Sensitivity

SPT5 Antibody recognizes endogenous levels of total SPT5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly803 of human SPT5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

DRB-sensitivity inducing factor (DSIF), a heterodimer composed of SPT4 and SPT5, is capable of both facilitating and inhibiting RNA polymerase II (RNAPII) activity (1-3). DSIF, together with NELF (Negative Elongation Factor), inhibits RNAPII elongation, resulting in promoter proximal pausing of RNAPII as it awaits additional signaling to resume transcription (4). The release of promoter proximal pausing is signaled through phosphorylation of the RNAPII C-terminal domain (CTD) and NELF by positive transcription elongation factor (P-TEFb) (5). P-TEFb also phosphorylates SPT5 at Thr4 within the evolutionarily conserved heptapeptide repeat motif. This phosphorylation event switches DSIF from a transcriptional repressor to an activator where it becomes a critical factor for transcriptional elongation (6,7).

1. Wada, T. et al. (1998) Genes Dev 12, 343-56.
2. Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67.
3. Gomes, N.P. et al. (2006) Genes Dev 20, 601-12.
4. Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
5. Buratowski, S. (2009) Mol Cell 36, 541-6.
6. Wada, T. et al. (1998) EMBO J 17, 7395-403.
7. Yamada, T. et al. (2006) Mol Cell 21, 227-37.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA, and 50% glycerol. Store at -20°C. Do not
aliquot the antibody.