ChIP-Grade Protein G Agarose Beads

• 产品编号：CST-9007S      品牌：CST       原厂货号：9007S
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 配体耦联磁珠 > Protein A&G 磁珠
• 应用分类：

描述：

ChIP级别的蛋白G琼脂糖微球是在染色质共沉淀（Chip）中用于小规模分离免疫复合物的基质。重组蛋白G以其截断形式与琼脂糖微球相偶联，表现出与很多物种（包括人类、兔子、小鼠、大鼠和养）的IgG亚型的高亲和力，并且可以与这些抗体共同应用于免疫沉淀实验。微球保存在包含BSA（500ug/ml)和经超声后的鲑鱼精子DNA（200ug/ml）的缓冲液中，可以在分离免疫复合物过程中减少蛋白和DNA的非特异性结合。在染色质共沉淀（Chip）中，作为亲和矩阵用于小规模分离免疫复合物。短暂涡旋管子以重悬微珠，每一个染色质免疫沉淀反应加入30ul微球。对于清洗微球和随后的洗脱免疫复合物，微球可以通过在微型离心机以6000rpm离心1分钟从溶液中分离。采用涡旋或者摇摆管子的方法用溶液重悬微珠。采用轻轻涡旋或者摇摆管子的方法用新的溶液重悬微珠。

原厂资料：

Description

ChiP-Grade Protein G Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from chromatin immunoprecipitations (ChIP assays). A truncated form of recombinant Protein G is covalently coupled to agarose beads. Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat and sheep) and can be used for immunoprecipitation assays with these antibodies. The beads are stored in buffer containing BSA (500 µg/ml) and sonicated salmon sperm DNA (200 µg/ml) to block non-specific binding of proteins and DNA during isolation of immunocomplexes.

Applications

Use as an affinity matrix for the small-scale isolation of immunocomplexes from chromatin immunoprecipitations (ChIP assays).

Directions for Use

Vortex tube briefly to resuspend the beads. Add 30 μl of bead slurry to each chromatin immunoprecipitation (ChIP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube.

注意事项：

Storage: Supplied in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA,
500 µg/ml BSA, 200 µg/ml sonicated salmon sperm DNA and
0.05% sodium azide. Store at 4ºC.