IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-8993S      品牌：CST       原厂货号：8993S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

IκBα (L35A5) Mouse mAb (识别氨基端抗原) (Alexa Fluor® 647偶联) 能够检测内源性IκBα总蛋白水平。此单克隆抗体通过GST-IκBα融合蛋白免疫动物制备，这种蛋白对应于人源IκBα蛋白氨基末端的肽段。CST的这种抗体与Alexa Fluor® 647荧光染料偶联，并且通过在人源细胞上的流式细胞分析进行严格内部检测。此抗体与未偶联IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814一样，具有同物种之间的交叉反应性。NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。IκBα 的Ser32 和 Ser36位点发生磷酸化后，蛋白酶体介导的降解使得激活型NF-κB释放并转位进入细胞核(3-7)。IκBα的磷酸化和Rel依赖的转录能够被很多胞外信号激活，包括炎症因子、生长因子和趋化因子。能够磷酸化IκB这些活性位点的激酶已经得到了鉴定(8)。

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

原厂资料：

Specificity / Sensitivity

IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total IκBα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino terminus of human IκBα protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2mg/ml BSA. Store at 4°C. Do not aliquot the antibody.
Protect from light. Do not freeze.