Cleaved PARP (Asp214) (D64E10) XP? Rabbit mAb (PE Conjugate)

• 产品编号：CST-8978S      品牌：CST       原厂货号：8978S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Cleaved PARP (Asp214) (D64E10) XP^® Rabbit mAb (PE Conjugate)兔单抗能够检测caspase酶切的人源PARP1蛋白大片段(89 kDa)的内源性水平。该抗体不能识别全长PARP1蛋白或其它PARP亚型蛋白。该单克隆抗体是采用合成的人源PARP蛋白Asp214周围残基相对应的肽段免疫动物而制备的。该CST抗体标记了藻红素(PE)，通过了内部测试可以直接用于人细胞的免疫荧光试验。该抗体与不带标记的Cleaved PARP (Asp214) (D64E10) XP^® Rabbit mAb兔单抗#5625有着相同的种属交叉反应。PARP，是核多聚(ADP-核糖) 聚合酶，分子量116kDa，参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3)，在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解，裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力；PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6）。

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5. Tewari, M. et al. (1995) Cell 81, 801-809.
6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

原厂资料：

Specificity / Sensitivity

Cleaved PARP (Asp214) (D64E10) XP^® Rabbit mAb (PE Conjugate) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved PARP (Asp214) (D64E10) XP^® Rabbit mAb #5625.

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
5. Tewari, M. et al. (1995) Cell 81, 801-809.
6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze