CLIP1/CLIP170 Antibody

• 产品编号：CST-8977S      品牌：CST       原厂货号：8977S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CLIP1/CLIP170抗体可以识别内源性总的CLIP1/CLIP170蛋白。多抗由合成肽段免疫动物产生，该肽段与人CLIP1/CLIP170蛋白羧基端肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。微管（MTs）是极化的中间丝，由α/βtubulin 异二聚体组成。MTs低速增长（mius）端位于中心体处，高速增长（plus）端则延展到细胞边缘。MT活性的调控对于多种细胞功能都是非常重要的，包括了细胞分裂，迁移，黏附，膜转运和细胞极化（1）。细胞质连接蛋白1/170 (CLIP1/CLIP170)位于MTs的plus端（2），与Rac1/cdc42效应蛋白IQGAP1结合。该复合物涉及建立细胞极性（3）。CLIP1/CLIP170也参与MT-依赖的细胞器转运（4），有丝分裂过程有效的微管-动粒结合需要PLK1和CK2磷酸化CLIP1/CLIP170（5）。

1. Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53.
2. Perez, F. et al. (1999) Cell 96, 517-27.
3. Fukata, M. et al. (2002) Cell 109, 873-85.
4. Lomakin, A.J. et al. (2011) Mol Biol Cell 22, 4029-37.
5. Li, H. et al. (2010) EMBO J 29, 2953-65.

原厂资料：

Specificity / Sensitivity

CLIP1/CLIP170 Antibody recognizes endogenous levels of total CLIP1/CLIP170 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLIP1/CLIP170 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Microtubules (MTs) are polarized filaments composed of α/β tubulin heterodimers. The slower growing (minus) ends of MTs are located at centrosomes, and the faster growing (plus) ends extend into the cell periphery. Regulation of MT dynamics is important for multiple cellular functions, including cell division, migration, adhesion, membrane trafficking, and polarity (reviewed in 1). 
 
 Cytoplasmic linker protein 1/170 (CLIP1/CLIP170) localizes to the plus ends of MTs (2), and binds to the Rac1/cdc42 effector protein IQGAP1. This complex is involved in establishing cell polarity (3). 
 
 CLIP1/CLIP170 also facilitates MT-dependent organelle transport (4), and phosphorylation of CLIP1/CLIP170 by PLK1 and CK2 is required for efficient microtubule–kinetochore attachments in mitosis (5).

1. Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53.
2. Perez, F. et al. (1999) Cell 96, 517-27.
3. Fukata, M. et al. (2002) Cell 109, 873-85.
4. Lomakin, A.J. et al. (2011) Mol Biol Cell 22, 4029-37.
5. Li, H. et al. (2010) EMBO J 29, 2953-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody