LAMTOR1/C11orf59 (D11H6) XP? Rabbit mAb

• 产品编号：CST-8975P      品牌：Cell Signaling Technology       原厂货号：8975P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LAMTOR1/C11orf59 (D11H6) XP ® Rabbit mAb兔单抗可以识别内源性的LAMTOR1/C11orf59蛋白。单克隆抗体是采用与人类 LAMTOR1/C11orf59 蛋白的Ala71周围残基相对应的合成肽段免疫动物生产的。mTORC1激酶复合物是调节细胞生长的一个关键组分（1,2）。其活性由能量水平，生长因子和氨基酸调节（3,4）。研究发现，RagA，RagB，RagC和RagD，这四个相关的GTPases与mTORC1中的raptor相互作用（1,2）。这些相互作用能有效激活mTORC1对氨基酸信号的响应，而且是这个过程必须的（1,2）。由LAMTOR1/C11orf59, LAMTOR2/ROBLD3, 和 LAMTOR3/MAPKSP1组成的蛋白复合体，已经被发现可以与四种Rag GTP酶相作用并招募它们至溶酶体表面（5）。

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. Sancak, Y. et al. (2010) Cell 141, 290-303.

原厂资料：

Specificity / Sensitivity

LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb recognizes endogenous levels of total LAMTOR1/C11orf59 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein.

Background

mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3 and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. Sancak, Y. et al. (2010) Cell 141, 290-303.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.