免疫染色,在样品上滴加ProLong® Gold Antifade Reagent with DAPI #8961并盖上盖玻片。为了防止褪色,将载玻片在黑暗中固化,室温,24小时,用透明漆(如指甲油)将盖玻片密封。下一步进行成像。ProLong® Gold Antifade Reagent with DAPI对光漂白作用有很强的抵抗力,本产品是预混合的可以直接使用,并且只引起很少或不产生荧光信号的猝灭。试剂在24小时内固化,封固的样品可以保存数月。本试剂与大多数染料一致,适用于多色标记检测。封固试剂中含有DAPI减少了一个单独的核复染步骤。4', 6 - 二脒基-2 - 苯基吲哚,卡匹帕明(DAPI)是一种蓝色荧光DNA染料,针对DNA小沟中的双股AT集群(1)。染料的一个分子结合dsDNA的3个碱基对,产生约20倍的荧光增强作用(2)。DAPI-DNA荧光水平与DNA含量成比例(3)。
原厂资料:
Directions for Use
Following immunostaining, cover samples with ProLong® Gold Antifade Reagent with DAPI #8961 and coverslip. To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.
Background
ProLong® Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
注意事项:
Storage: ProLong® Gold Antifade Reagent is stable for 6
months when stored protected from light, at or below -20ºC.