Phospho-Tyrosine (P-Tyr-1000) MultiMab? Rabbit mAb mix

• 产品编号：CST-8954S      品牌：CST       原厂货号：8954S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb兔单抗能够识别大量酪氨酸磷酸化的蛋白和多肽。该抗体不会与丝氨酸磷酸化或苏氨酸磷酸化的蛋白或多肽产生交叉反应。该单克隆抗体是由含有磷酸化酪氨酸的多肽免疫动物制备。酪氨酸磷酸化在细胞信号转导中扮演重要角色（1）。研究发现，在癌症中，失调的酪氨酸激酶活性会产生不当的增殖和存活信号从而导致恶性肿瘤以及肿瘤形成（2）。针对磷酸化酪氨酸(3,4)的特异性抗体是进行这些研究时非常宝贵的试剂。Cell Signaling Technology研发的磷酸化酪氨酸单克隆抗体具有卓越的灵敏度，可用于研究高通量药物开发中的酪氨酸磷酸化并监测酪氨酸激酶活性。

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

原厂资料：

Specificity / Sensitivity

Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody