Phospho-NDRG1 (Thr346) (D98G11) XP? Rabbit mAb (HRP Conjugate)

• 产品编号：CST-8947S      品牌：CST       原厂货号：8947S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb（HRP偶联）可以识别Thr346被磷酸化的内源性NDRG1蛋白。另外此抗体和Thr356及Thr366被磷酸化的NDRG1蛋白貌似有交叉反应。合成对应小鼠NDRG1蛋白的邻近Thr346氨基酸残基序列的肽段，免疫动物获得单克隆抗体。Cell Signaling Technology^® 公司抗体通过其胺基与辣根过氧化物酶基团（HRP）的碳水化合物组偶联。此HRP偶联抗体与未偶联的Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb #5482有同样的物种反应性。N-myc下游调控gene1（NDRG1），也被称为Cap43，Drg1，RTP/rit42和Proxy-1，是NDRG家族的一员，该家族包含的4个成员（NDRG1-4）在生长，分化和细胞存活过程中发挥作用（1-5）。广泛表达的NDRG1并对多种压力信号发生应激反应，包括DNA损伤（4），缺氧（5），和镍及钙浓度升高（2）。NDRG1在N-myc缺陷小鼠中表达升高，能够被N-和c-myc负调控（1-6）。在DNA损伤过程中，NDRG1通过p53信号激活，并且是p53介导的凋亡过程中所必须的（4,7）。研究显示，NDRG1通过促进分化，抑制生长，调控血管生成和肿瘤转移等进程中发挥功能（3,4,6,8,9）。NDRG1基因发生无义突变后会引发遗传性运动和感觉神经病病变-Lom(HMSNL),该结论由证明NDRG1在维持髓鞘和轴突存活的研究支持（10,11）。NDRG1在肥大细胞成熟过程中表达上调，它的缺失会导致衰减的过敏反应（12）。NDRG1和NDRG2都是SGK1的底物，尽管SGK1介导的磷酸化的生理性作用尚未可知（13）。Cell Signaling Technology (CST)使用PhosphoScan^®, CST's LC-MS/MS 平台以发现蛋白修饰点，设计了Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb。Thr346的磷酸化是通过一个Akt底物抗体发现的，并在多种细胞系中发现可以被胰岛素刺激诱导表达。请访问www.phosphosite.org中的CST's修饰位点网站，PhosphoSitePlus^®，以获得更多的信息。

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

原厂资料：

Specificity / Sensitivity

Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb (HRP Conjugate) detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr346 of mouse NDRG1 protein.

Description

This Cell Signaling Technology^® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb #5482.

Background

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.Phospho-NDRG1 (Thr346) (D98G11) XP^® Rabbit mAb is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan^®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Thr346 was discovered using an Akt substrate antibody and was shown to be induced by insulin treatment in multiple cell lines. Please visit PhosphoSitePlus^®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody.