Human Granulocyte Colony Stimulating Factor (hG-CSF)

• 产品编号：CST-8930LC      品牌：CST       原厂货号：8930LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > CSF集落刺激因子
• 应用分类：

描述：

CST公司生产的人源重组蛋白G-CSF (hG-CSF) Thr31-Pro204 (Accession #NP_757373)是从人源293细胞表达而来。重组的hG-CSF蛋白在末端没有标签，未糖基化蛋白的分子量据推算为18,986Da。DTT-降解蛋白和未降解的蛋白作为一个18kDa的多肽而转移。重组hG-CSF蛋白的N-末端TPLGP的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hG-CSF通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hG-CSF 的生物活性是通过M-NFS-60细胞的增值实验来确定的。每个批次的ED50值在20-150 pg/ml之间。内毒素含量：小于0.01 ng /1 μg hG-CSF。

有载体: 每微克hG-CSF蛋白溶解在包含 250 mM NaCl 和 20 μg BSA的40 mM 磷酸盐（pH 4.0）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hG-CSF蛋白溶解在包含 250 mM NaCl 的40 mM 磷酸盐（pH 4.0）溶液中，并通过 0.22 μm 滤膜冻干。

G-CSF是造血因子，对噬中性粒细胞发育、生存和从骨髓的释放有重要的作用(1-4)。巨噬细胞和单核细胞是G-CSF最重要的生产者(3)，内皮细胞、纤维原细胞和神经元细胞受到炎症的刺激后也能产生G-CSF(3)。G-CSF 在嗜中性粒细胞和神经元中抑制细胞凋亡(4,5)。G-CSF刺激神经元原细胞的增殖和分化(5)。G-CSF结合到 G-CSFR上诱导受体的二聚化并激活Jak1/2将酪氨酸磷酸化(3,6)。信号通路经过Stat3, ERK, p38, 和 Akt信号分子的转导 (5,6)。在人和小鼠中缺少了G-CSF功能或者其受体引起是噬中性白血球减少症(7,8)。

原厂资料：

Source / Purification

Recombinant human G-CSF (hG-CSF) Thr31-Pro204 (Accession #NP_757373) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hG-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,986. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal TPLGP of recombinant hG-CSF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hG-CSF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hG-CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 20-150 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg hG-CSF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl and 20 μg BSA per 1 μg hG-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 40 mM phosphate pH 4.0 containing 250 mM NaCl.

Background

G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.