Human Macrophage Colony Stimulating Factor (hM-CSF)

• 产品编号：CST-8929SF      品牌：CST       原厂货号：8929SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > CSF集落刺激因子
• 应用分类：

描述：

CST公司生产的人源融合蛋白M-CSF (hM-CSF) Glu33-Ser190 (Accession #P09603-3)是从人源293细胞表达而来。重组的hM-CSF 蛋白在末端没有标签，未糖基化蛋白的分子量据推算为18,403Da。DTT-降解蛋白迁移时是作为一个16-26kDa 的多肽而转移，未降解的蛋白在胱氨酸连接的二聚体中作为一个34-40kDa的蛋白而转移。SDS胶中不同的分子量是因为糖基化的原因。重组hM-CSF 蛋白的N-末端EEVSE的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hM-CSF通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。融合蛋白hM-CSF的生物活性是通过M-NFS-60 细胞的增值实验来确定的。每个批次的ED50值在0.5-2.0 ng/ml之间。内毒素含量：小于0.01 ng /1 μg hM-CSF。

有载体: 每微克hM-CSF蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hM-CSF蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

巨噬细胞克隆刺激因子 (M-CSF)由纤维原细胞、内皮细胞、基质细胞、巨噬细胞、造骨细胞和其他的一些细胞产生(1)。M-CSF是单核细胞和巨噬细胞生长和分化所必需的 (1,2)。M-CSF使巨噬细胞极化为 M2 表型，产生抗炎症因子IL-10，而不是M1表型产生炎症因子。M-CSF也可招募单核细胞并通过诱导VEGF的产生而增强血管生成(1,2)。M-CSF结合到它们的受体(CSF1R)上，下游参与的信号分子有 PI3K/Akt, ERK 和 STATs 1, 3, 5 (1,3)。M-CSF表达的增加对癌症的发展有很大的贡献 ，同时血浆中 M-CSF水平的升高与类风湿性关节炎有关(1,4,5)。

原厂资料：

Source / Purification

Recombinant human M-CSF (hM-CSF) Glu33-Ser190 (Accession #P09603-3) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,403. DTT-reduced protein migrates as a 16-26 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34-40 kDa protein. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino-terminal EEVSE of recombinant hM-CSF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hM-CSF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hM-CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-2.0 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hM-CSF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor (CSF1R); downstream signaling involves PI3K/Akt, ERK and STATs 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression and a higher plasma M-CSF level is associated with rheumatoid arthritis (1,4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.