Human Interleukin-17A (hIL-17A)

• 产品编号：CST-8928SF      品牌：CST       原厂货号：8928SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

CST公司生产的人源融合蛋白IL-17A (hIL-17A) Ile20-Ala155 (Accession #NP_002181)是从人源293细胞表达而来。重组的hIL-17A 蛋白在末端没有标签，其糖基化蛋白的分子量据推算为15,535Da。DTT-降解蛋白迁移时是作为一个16-24kDa 的多肽而转移，在SDS胶中显示不同的分子量是因为其糖基化。未降解的蛋白在胱氨酸连接的二聚体中作为一个28-37kDa的蛋白而转移。重组hIL-17A 蛋白的N-末端IVKAG的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-17A通过SDS-PAGE检测，纯度大于97%，所有批次的纯度均高于97%。hIL-17A 融合蛋白的生物活性是通过诱导人成纤维细胞产生IL-6能力来确定的。每个批次的ED50值在1.5 - 3.5 ng/ml之间。内毒素含量：小于0.01 ng /1 μg hIL-17A。

有载体: 每微克hIL-17A蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-17A蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-17A 是通过胱氨酸连接的同型二聚体，通过独特的CD4+ T细胞系产生的促炎症细胞因子(1,2)。IL-17A 刺激促炎症因子IL-1β, TNF-α,和 IL-6的产生。IL-17A 也能够诱导产生噬中性粒细胞趋化剂如IL-8, CXCL1和 CXCL6，这样就将天然免疫和获得性免疫连接起来(1,2)。IL-17A跟粘膜抵抗细菌侵染有密切的联系(1,3) 同时也在自身免疫混乱过程中有潜在的功能(1,4)。IL-17A 通过结合到 IL-17RA发挥作用(5)。IL-17A与IL-17RA的结合通过激活Erk1/2 MAP激酶, PI3K/Akt, p38, 和 NF-κB 信号通路诱导产生细胞因子，趋化因子和一些相关的蛋白(3,4, 6)。它也能够磷酸化一些 Jaks 和 Stats 家族的蛋白。

原厂资料：

Source / Purification

Recombinant human IL-17A (hIL-17A) Ile20-Ala155 (Accession #NP_002181) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-17A contains no "tags" and the nonglycosylated protein has a calculated MW of 15,535. DTT-reduced protein migrates as a 16-24 kDa polypeptide. Heterogeneity in SDS PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 28-37 kDa protein. The expected amino-terminal IVKAG of recombinant hIL-17A was verified by amino acid sequencing.

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A. All lots are greater than 97% pure

Bioactivity

The bioactivity of recombinant hIL-17A was determined by its ability to induce IL-6 production by primary human fibroblasts. The ED50 of each lot is between 1.5 - 3.5 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-17A.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17A. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4, 6). Phosphorylation of some Jaks and Stats has been observed.

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.