Human Interferon-α1 (hIFN-α1)

• 产品编号：CST-8927SC      品牌：CST       原厂货号：8927SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > Interferon 干扰素
• 应用分类：

描述：

人源融合蛋白IFN-α1 (hIFN-α1) Cys24 - Asp189 (Accession # NP_076918)是由CST公司生产，此蛋白是在大肠杆菌中表达的。重组的hIFN-α1 蛋白在N-末端没有Met，其分子量据推算为19,386Da。DTT-降解蛋白迁移时是作为一个18 kDa 的多肽而转移，未降解的蛋白由于有cystines存在所以迁移时是作为一个14 kDa 的多肽而转移。重组hIFN-α1 蛋白的N-末端CDLPE的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIFN-α1通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hIFN-α1 的生物活性是通过病毒保护实验来确定的。每个批次的ED50值在20 - 60 pg/ml之间。内毒素含量小于0.01 ng/1 μg hIFN-α1。

有载体: 每微克hIFN-α1蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIFN-α1蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

干扰素-α1 是I型IFN中的一员 (1)， 此家族以抗病毒活性而著称。受到病毒感染后，大多数有核细胞能产生一种或多种I型IFNs(2)。分泌I型IFN后对周围未受到干扰的细胞进行诱导保护性响应。I型IFNs 也能增强病毒诱导的细胞凋亡(3)。其它IFN-α1活性包括增进树状细胞的成熟和细胞毒素T细胞的活性(4)。IFN-α1 结合到IFN-αR1 和IFN-αR2 异源二聚体上(1)。 细胞内通过Jak/Stat的信号通路已经得到深入的研究(3)。 然而, PI3K, ERK 和 p38 激酶通路也有参与(5)。IFNs抗病毒的活性被应用于处理病毒感染(4)。I型IFNs在自身免疫性疾病中也有重要的作用(6)。

原厂资料：

Source / Purification

Recombinant human IFN-α1 (hIFN-α1) Cys24 - Asp189 (Accession # NP_076918) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIFN-α1 does not have a Met on the amino terminus and has a calculated MW of 19,386. DTT-reduced protein migrates as an 18 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal CDLPE of recombinant hIFN-α1 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIFN-α1. All lots are greater than 98% pure.

Bioactivity

The bioactivity of hIFN-α1 was determined in a virus protection assay. The ED50 of each lot is between 20 - 60 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIFN-α1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIFN-α1. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Interferon-α1 is a member of the Type I IFN (1) family best known for their antiviral activity. Most nucleated cells produce one or more Type I IFNs in response to viral infection (2). Secreted Type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFN-α1 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFN-α1 binds to the IFN-αR1 and IFN-αR2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.