Human Stem Cell Factor (hSCF)

• 产品编号：CST-8925SF      品牌：CST       原厂货号：8925SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > Stem Cell Factor 干细胞因子
• 应用分类：

描述：

CST公司的重组人SCF(hSCF) 蛋白Glu26-Ala189 (Accession #P21583)在人293细胞表达获得。重组hSCF蛋白不带标签，非糖基化蛋白分子量是18,458。DTT-还原和非还原蛋白迁移位于22-35 kDa。SDS-PAGE中低迁移速率和蛋白异质性是由糖基化引起的。重组hSCF氨基端EGICR通过蛋白质测序验证。重组hSC蛋白F经过还原（+）和非还原（-）SDS-PAGE验证，所有批次纯度大于98%。重组hSCF的生物活性在M-07e细胞增殖实验中检测，每一批次的ED50在2-6ng/ml之间。1ug hSCF含有内毒素量低于0.01ng。包含载体蛋白形式：PBS缓冲液，pH 7.2 包含20 μg BSA 每1 μg hSCF用0.22um滤膜过滤后冻干。不含载体蛋白形式：pH 7.2 PBS经过0.22um滤膜过滤后冻干。SCF存在于内皮细胞，成纤维细胞，角质化细胞，肠上皮细胞和癌细胞（1,2）。SCF对于造血作用和肥大细胞的分化非常重要，并且也在其他类型细胞的生存等调控中扮演角色（1）。一些肿瘤细胞的增殖和侵袭受SCF蛋白起始（3）。肿瘤来源的SCF似乎涉及骨髓来源的抑制细胞的增殖，这种抑制细胞会限制肿瘤-侵润－T细胞的扩增（4）。SCF在肿瘤微环境中也有其他作用（2）。SCF可以以可溶蛋白状态或嵌膜蛋白状态存在，它的存在形式决定于剪切或蛋白水解释放的不同异构体（1）。SCF可以与酪氨酸激酶受体c-Kit结合，随后激活Akt, Erk, JNK和p38 信号通路 (5,6)。

原厂资料：

Source / Purification

Recombinant human SCF (hSCF) Glu26-Ala189 (Accession #P21583) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,458. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal EGICR of recombinant hSCF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSCF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hSCF was determined in a M-07e cell proliferation assay. The ED50 of each lot is between 2-6 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hSCF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hSCF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.