Mouse Interleukin-3 (mIL-3)

• 产品编号：CST-8923SC      品牌：CST       原厂货号：8923SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白白介素-3 (mIL-3) Ala27-Cys166 (Accession # NM_010556)是从大肠杆菌中表达而来。重组的mIL-3蛋白在末端没有标签，其分子量据推算为15,674Da。DTT-还原蛋白作为一个16kDa的蛋白而转移，未还原的蛋白由于具有分子内的胱氨酸而作为14kDa多肽迁移。重组mIL-3蛋白的N-末端ASISG的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mIL-3通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mIL-3的生物活性是通过MC/9和BaF3细胞的增殖实验确定的。在MC/9细胞中每个批次的ED50值在2-50 pg/ml之间，在BaF3细胞中每个批次的ED50值在20-90pg/ml之间。内毒素含量：小于0.01 ng /1 μg mIL-3。

有载体: 每微克mIL-3蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIL-3蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-3是通过T细胞、肌肉细胞和噬曙红细胞而产生的(1)。目标细胞包括造血原细胞、嗜中性粒细胞、巨噬细胞、肌肉细胞、噬曙红细胞、淋巴细胞和色调微红细胞(1)。IL-3支持生长和分化并且作为很多类型细胞的培养基的添加成分(1)。IL-3受体是一个异源二聚体包含IL-3 特异的α-链和普遍存在的β-链, βc, 也被GM-CSF 和 IL-5所利用 (1)。IL-3特异性的βIL-3-链可替换βc并参与到IL-3的结合中 (1,2)。IL-3结合到其同类的受体上诱导了Jak2的激活,磷酸化多个Stats (1,3,5,6), 和 PI3K/Akt信号通路(1)。IL-3在气管炎症引发的哮喘过程中有重要的作用(3,4,5)。

原厂资料：

Source / Purification

Recombinant mouse interleukin-3 (mIL-3) Ala27-Cys166 (Accession # NM_010556) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-3 does not have a Met on the amino terminus and has a calculated MW of 15,674. DTT-reduced protein migrates as a 16 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal ASISG of recombinant mIL-3 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-3 was determined in MC/9 and BaF3 cell proliferation assays. The ED50 of each lot is between 2-50 pg/ml in MC/9 cells and 20-90 pg/mL in BaF3 cells.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-3.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.