Human Interleukin-21 (hIL-21)

• 产品编号：CST-8920SC      品牌：CST       原厂货号：8920SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的人源重组蛋白IL-21 (hIL-21) Gln30-Ser155 (Accession # NM_021803)是从人源293细胞表达而来。重组的hIL-21蛋白在末端没有标签，未糖基化的蛋白分子量据推算为15,463Da。DTT-降解蛋白和未降解蛋白作为一个16kDa的蛋白而转移。未降解的蛋白具有稍高的迁移率与分子内的胱氨酸有关。重组hIL-21蛋白的N-末端QGQDR的序列通过测序得到。

6 μg 还原 (+) 和 非还原的(-) hIL-21通过SDS-PAGE检测，纯度大于98%。

在还原的hIL-21，只有9%的组分会在Arg114位后发生切割而产生6kD和10kD的多肽。

重组蛋白hIL-21 的生物活性是通过诱导NK-92细胞产生IFN-γ的能力实验确定的。每个批次的ED50值在1.0-6.0 ng/ml之间。内毒素含量：小于0.01 ng /1 μg hIL-21。

有载体: 每微克hIL-21蛋白溶解在 包含 20 μg BSA的PBS（pH 7.2 ）的溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-21蛋白溶解在PBS（pH 7.2 ）的溶液中，并通过 0.22 μm 滤膜冻干。

IL-21 是由Th17、T 卵泡助细胞(Tfh) 和NKT细胞产生 (1-3)。靶标包括 T细胞, B细胞, NK 细胞和树突状细胞(1-3,5)。IL-21 诱导NK细胞的增殖和激活，从而上调IFN-γ 的产生和细胞毒素的活性(1,3,4)。IL-21增加CD40起源的B细胞的增殖和存活(1,2) 并且在血浆细胞的分化工程中有非常重要的功能(2)。IL-21 结合到由IL-21Rα和普遍的 γ 链—γc组成的复合体上。IL-21的结合激活了Jak1/Jak3, Stat1, Stat3 和 Stat5 信号通路. IL-21的结合也能激活MAP 激酶和PI3K/Akt通路。

原厂资料：

Source / Purification

Recombinant human IL-21 (hIL-21) Gln30-Ser155 (Accession # NM_021803) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-21 contains no "tags" and the nonglycosylated protein has a calculated MW of 15,463. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides, with non-reduced having slightly greater mobility due to intramolecular cystines. The expected amino-terminal QGQDR of recombinant hIL-21 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-21. Less than 9% is nicked after Arg114 to generate 6 kDa and 10 kDa polypeptides observed in reduced (+) hIL-21.

Bioactivity

The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50 of each lot is between 1.0-6.0 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-21.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-21. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40 primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.