Human Interleukin-4 (hIL-4)

• 产品编号：CST-8919SF      品牌：CST       原厂货号：8919SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司生产的人源重组蛋白IL-4 (hIL-4) His25-Ser153 (Accession #AF395008)是从人源293细胞表达而来。重组的hIL-4蛋白在N-末端没有Met标签，未糖基化的蛋白分子量据推算为14,963。DTT-降解蛋白和未降解蛋白由于糖基化的存在而作为一个26kDa的蛋白转移。未降解的蛋白的迁移率稍高是因为分子内的胱氨酸存在。重组hIL-4蛋白的N-末端HKCDI的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-4通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hIL-4的生物活性是通过TF-1细胞的增殖实验确定的。每个批次的ED50值在80-250pg/ml之间。内毒素含量：小于0.01 ng /1 μg hIL-4。

有载体: 每微克hIL-4蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-4蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-4通过T 细胞, NK T 细胞, γδ 细胞和肌肉细胞产生(1)。目标细胞包括B 细胞, T 细胞和巨噬细胞(1)。IL-4 诱导T细胞分化为TH2表型。IL-4也能促进B细胞增殖、抗体类型转换、以及其它TH2细胞分泌因子—IL-5和IL-9细胞因子的表达。IL-4诱导TH2的极化，对体液免疫病原菌的发展有重要的作用(1)，同时也参与到敏感症和哮喘的发病过程中(2)。IL-4 结合到两个不同的受体上, type I 受体和type II 受体。Type I 受体是异源二聚体包含IL-4Rα 链和普遍存在的 gamma chain, γc (3,4)。Type II 受体, 与IL-13受体共享, 是IL-4Rα 和 IL-13Rα1的二聚体。通过type I受体进行传递的信号能激活 Jak1/Stat6, Jak3 和 PI3K/Akt通路 (3)。type II 受体能激活 Jak1/Stat6和Tyk2/Stat3通路(3)。

原厂资料：

Source / Purification

Recombinant human IL-4 (hIL-4) His25-Ser153 (Accession #AF395008) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-4 does not have a Met on the amino terminus and the nonglycosylated protein has a calculated MW of 14,963. DTT-reduced and non-reduced protein migrate as larger 20 kDa polypeptides due to glycosylation, with non-reduced having slightly greater mobility due to an intramolecular cystine. The expected amino-terminal HKCDI of recombinant hIL-4 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-4. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 80-250 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-4.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-4. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-4 is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces differentiation of naive T cells into the TH2 phenotype. IL-4 also promotes B cell proliferation, antibody isotype switching and expression of other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (2). IL-4 binds to two distinct receptors, the type I receptor and type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (3,4). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (3). The type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.